Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
Anal Chem. 2012 May 15;84(10):4622-7. doi: 10.1021/ac300577d. Epub 2012 Apr 24.
We have developed a surface-enhanced Raman scattering (SERS)-active substrate based on gold nanoparticle-decorated chemical vapor deposition (CVD)-growth graphene and used it for multiplexing detection of DNA. Due to the combination of gold nanoparticles and graphene, the Raman signals of dye were dramatically enhanced by this novel substrate. With the gold nanoparticles, DNA capture probes could be easily assembled on the surface of graphene films which have a drawback to directly immobilize DNA. This platform exhibits extraordinarily high sensitivity and excellent specificity for DNA detection. A detection limit as low as 10 pM is obtained. Importantly, two different DNA targets could be detected simultaneously on the same substrate just using one light source.
我们开发了一种基于金纳米粒子修饰的化学气相沉积（CVD）生长石墨烯的表面增强拉曼散射（SERS）活性基底，并将其用于 DNA 的多重检测。由于金纳米粒子和石墨烯的结合，这种新型基底极大地增强了染料的拉曼信号。通过金纳米粒子，DNA 捕获探针可以很容易地组装在石墨烯薄膜的表面，而石墨烯薄膜直接固定 DNA 存在缺点。该平台对 DNA 检测表现出极高的灵敏度和优异的特异性。检测限低至 10 pM。重要的是，只需使用一个光源，就可以在同一基底上同时检测到两种不同的 DNA 靶标。
