Institute of Legal Medicine, Innsbruck Medical University, Innsbruck, Austria.
Forensic Sci Int Genet. 2014 Jan;8(1):90-100. doi: 10.1016/j.fsigen.2013.07.002. Epub 2013 Sep 7.
To gain general acceptance forensic DNA testing in animals needs to improve standardization of analysis methods and data interpretation. Recently, the International Society of Forensic Genetics (ISFG) took particular care of this topic by publishing recommendations for forensic non-human DNA analysis following the successful example of human DNA analysis in order to provide a basis for harmonization of the still existing inter-laboratory variability. By following these recommendations we demonstrate the performance of two short tandem repeat (STR) multiplexes for forensic identity testing of canine biological material. Thirteen STRs and two sex-specific markers were selected and validated according to the ISFG guidelines. Population genetic parameters were calculated based on 295 dog samples collected in Austria (124) and Germany (171). A repeat-based nomenclature of the mainly tetrameric STRs and corresponding allelic ladders are presented. All 146 different alleles included in the ladders were sequenced for correct allele calling. Additionally, a canine cell line (DH82-D3167) was evaluated as standard reference material.
为了使法医 DNA 检测在动物中的应用得到广泛认可，需要提高分析方法和数据解释的标准化程度。最近，国际法医遗传学学会（ISFG）特别关注这个话题，效仿人类 DNA 分析成功发布了法医非人类 DNA 分析的建议，旨在为协调目前仍然存在的实验室间变异性提供依据。通过遵循这些建议，我们展示了两种短串联重复（STR）多重扩增试剂盒在犬类生物材料法医身份鉴定中的性能。根据 ISFG 指南，选择并验证了 13 个 STR 和两个性别特异性标记。基于在奥地利（124 只）和德国（171 只）收集的 295 个犬样本，计算了群体遗传参数。本文提出了基于重复序列的主要四聚体 STR 命名法及其对应的等位基因梯。对梯中的 146 个不同等位基因进行测序，以正确进行等位基因的命名。此外，还评估了犬类细胞系（DH82-D3167）作为标准参考材料。
