小鼠组蛋白编码基因簇的结构。

Structure of a mouse histone-encoding gene cluster.

作者信息

Gruber A, Streit A, Reist M, Benninger P, Böhni R, Schümperli D

机构信息

Abteilung für Entwicklungsbiologie, Zoologisches Institut der Universität Bern, Switzerland.

出版信息

Gene. 1990 Nov 15;95(2):303-4. doi: 10.1016/0378-1119(90)90377-4.

DOI:10.1016/0378-1119(90)90377-4

PMID:2249786

Abstract

In addition to a previously described histone (H)-encoding H4 gene [Meier et al., Nucleic Acids Res. 17 (1989) 795], the mouse genomic DNA clone 53 contains two H3 genes, one functional and one partially deleted H2A gene, and one H2B gene. Clone 53 overlaps for 3 kb with MH143, another previously isolated mouse H-encoding clone [Yang et al., J. Biol. Chem. 262 (1987) 17118-17125], thus defining a 32-kb region of mouse chromosome 13 with a total of seven H-encoding genes. We have determined the nucleotide sequences and transcription start points of two genes coding for the H2A.1 and H3.2 proteins.

摘要

除了先前描述的编码组蛋白（H）的H4基因[迈尔等人，《核酸研究》17（1989）795]外，小鼠基因组DNA克隆53还包含两个H3基因、一个功能正常的H2A基因和一个部分缺失的H2A基因，以及一个H2B基因。克隆53与另一个先前分离的小鼠H编码克隆MH143重叠3 kb[杨等人，《生物化学杂志》262（1987）17118 - 17125]，从而确定了小鼠13号染色体上一个32 kb的区域，该区域共有七个H编码基因。我们已经确定了编码H2A.1和H3.2蛋白的两个基因的核苷酸序列和转录起始点。