Ehinger A, Denison S H, May G S
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Mol Gen Genet. 1990 Jul;222(2-3):416-24. doi: 10.1007/BF00633848.
The core histone gene family of Aspergillus nidulans was characterized. The H2A, H2B and H3 genes are unique in the A. nidulans genome. In contrast there are two H4 genes, H4.1 and H4.2. As previously reported for the H2A gene (May and Morris 1987) introns also interrupt the other core histone genes. The H2B gene, like the H2A gene, is interrupted by three introns, the H3 and H4.1 gene are each interrupted by two introns and the H4.2 gene contains one intron. The position of the single intron in H4.2 is the same as that the first intron of the H4.1 gene. The H2A and H2B genes are arranged as a gene pair separated by approximately 600 bp and are divergently transcribed. The H3 and H4.1 genes are similarly arranged and are separated by approximately 800 bp. The H4.2 gene is not closely linked to either the H2A-H2B or H3-H4.1 gene pairs. Using pulse field gel electrophoresis an electrophoretic karyotype was established for A. nidulans. This karyotype was used to assign the H3-H4.1 gene pair and the H4.2 gene to linkage group VIII and the H2A-H2B gene pair to either linkage group III or VI. The abundance of each of the histone messenger RNAs was determined to be cell cycle regulated but the abundance of the H4.2 mRNA appears to be regulated differently from the others.
对构巢曲霉的核心组蛋白基因家族进行了表征。H2A、H2B和H3基因在构巢曲霉基因组中是独特的。相比之下，有两个H4基因，即H4.1和H4.2。如先前关于H2A基因的报道（梅和莫里斯，1987年），内含子也会中断其他核心组蛋白基因。H2B基因与H2A基因一样，被三个内含子中断，H3和H4.1基因各自被两个内含子中断，而H4.2基因包含一个内含子。H4.2中单个内含子的位置与H4.1基因的第一个内含子位置相同。H2A和H2B基因作为一对基因排列，间隔约600 bp，且转录方向相反。H3和H4.1基因的排列方式类似，间隔约800 bp。H4.2基因与H2A - H2B或H3 - H4.1基因对均无紧密连锁。使用脉冲场凝胶电泳为构巢曲霉建立了电泳核型。该核型用于将H3 - H4.1基因对和H4.2基因定位于连锁群VIII，将H2A - H2B基因对定位于连锁群III或VI。已确定每种组蛋白信使RNA的丰度受细胞周期调控，但H4.2 mRNA的丰度似乎与其他的调控方式不同。
