Ratech H
Fox Chase Cancer Center, Department of Pathology, Philadelphia, PA.
Hum Pathol. 1990 Dec;21(12):1275-82. doi: 10.1016/s0046-8177(06)80042-0.
Ten cases of reactive follicular hyperplasia and 31 cases of B-cell non-Hodgkin's malignant lymphoma were studied using multiparameter flow cytometry. A bimodal distribution for HLA-DR expression, but not for surface immunoglobulin or B cell-specific antigens CD19 and CD20, was observed commonly in mixed cell type and infrequently in non-mixed cell type B-cell malignant lymphomas. On the basis of HLA-DR distribution alone, 31 cases of B-cell malignant lymphomas of low, intermediate, and high grades could be separated into mixed and non-mixed cell types, with only two misclassifications (P = 0.0001). Exceptionally, one case of malignant lymphoma, follicular and diffuse, mixed-cell type had a unimodal HLA-DR distribution, and one case of malignant lymphoma, diffuse, large noncleaved cell type had a bimodal HLA-DR distribution. In all cases of malignant lymphoma, follicular, mixed-cell type studied, low HLA-DR was correlated with small cells, and high HLA-DR was correlated with large cells. In contrast, HLA-DR expression and cell size were not as directly correlated in cases of malignant lymphoma, diffuse, mixed-cell type. These observations suggest that most, but not all, cases of B-cell malignant lymphomas of the mixed cell type can be separated from other B-cell lymphomas on the basis of HLA-DR distribution.
采用多参数流式细胞术对10例反应性滤泡增生和31例B细胞非霍奇金恶性淋巴瘤进行了研究。在混合细胞型B细胞恶性淋巴瘤中，常见HLA-DR表达呈双峰分布，而表面免疫球蛋白或B细胞特异性抗原CD19和CD20则不然，在非混合细胞型B细胞恶性淋巴瘤中则很少见。仅根据HLA-DR分布，31例低、中、高分级的B细胞恶性淋巴瘤可分为混合细胞型和非混合细胞型，仅有两例假分类（P = 0.0001）。例外的是，1例滤泡性和弥漫性混合细胞型恶性淋巴瘤的HLA-DR分布为单峰，1例弥漫性大无裂细胞型恶性淋巴瘤的HLA-DR分布为双峰。在所有研究的滤泡性混合细胞型恶性淋巴瘤病例中，低HLA-DR与小细胞相关，高HLA-DR与大细胞相关。相比之下，在弥漫性混合细胞型恶性淋巴瘤病例中，HLA-DR表达与细胞大小的相关性不那么直接。这些观察结果表明，大多数（但不是全部）混合细胞型B细胞恶性淋巴瘤病例可根据HLA-DR分布与其他B细胞淋巴瘤区分开来。
