Takahashi H, Fujita S, Okabe H, Tsuda N, Tezuka F
Department of Oral Pathology, Nagasaki University School of Dentistry, Japan.
Pathol Res Pract. 1993 Apr;189(3):300-11. doi: 10.1016/S0344-0338(11)80514-5.
Fifty cases of extranodal non-Hodgkin's lymphoma arising in the oral cavity were reclassified using the updated Kiel classification. In order to determine the antigenic phenotype of the proliferating cells in oral lymphoma, we used a panel of paraffin effective antibodies that are known to react with lymphocyte- and histiocyte-associated antigens. The median age of the patients was 53 years, with a male predominance (M:F = 1.9: 1). The great majority of oral non-Hodgkin's lymphomas were B-cell lymphomas. There were 12 low-grade B-cell lymphomas (comprising one mucosa-associated lymphoid tissue, four centrocytic and seven centroblastic-centrocytic lymphomas) and 25 high-grade tumors (comprising 17 centroblastic, two immunoblastic, two Burkitt's and four lymphoblastic lymphomas). All 37 B-cell malignancies showed reactivity for L 26 and KiB 3. A monotypic immunoglobulin staining pattern, as revealed by light chain restriction, was found in 21 cases (57%) of the non-Hodgkin's lymphomas confirming their B-cell origin. Furthermore, monotypic staining for kappa-chain predominated (16/21 kappa, 5/21 lambda). Only a small number (6 cases) was of T-cell lineage and all cases showed positive reaction for UCHL 1, MT 1 and DFT 1. In one of six T-cell lymphomas, Ber-H 2 positive anaplastic large cell lymphoma was detected. Such a case was documented for the first time in the primary extranodal non-Hodgkin's lymphoma of the oral cavity. Five cases could be assigned with certainty to the histiocytic system. These cases were positive for cathepsin D and KP 1 LN 3, which recognized Ia (HLA-DR) antigens, was demonstrated most frequently in high-grade B-cell lymphomas, T-cell lymphomas and true histiocytic lymphomas.
采用更新后的基尔分类法对50例原发于口腔的结外非霍奇金淋巴瘤进行了重新分类。为了确定口腔淋巴瘤中增殖细胞的抗原表型，我们使用了一组已知能与淋巴细胞和组织细胞相关抗原发生反应的石蜡有效抗体。患者的中位年龄为53岁，男性占优势（男:女 = 1.9:1）。绝大多数口腔非霍奇金淋巴瘤为B细胞淋巴瘤。其中有12例低级别B细胞淋巴瘤（包括1例黏膜相关淋巴组织淋巴瘤、4例中心细胞性淋巴瘤和7例中心母细胞 - 中心细胞性淋巴瘤）以及25例高级别肿瘤（包括17例中心母细胞性淋巴瘤、2例免疫母细胞性淋巴瘤、2例伯基特淋巴瘤和4例淋巴母细胞性淋巴瘤）。所有37例B细胞恶性肿瘤对L26和KiB3均呈阳性反应。通过轻链限制显示，在21例（57%）非霍奇金淋巴瘤中发现了单型免疫球蛋白染色模式，证实了它们的B细胞起源。此外，κ链的单型染色占主导（21例中有16例κ链，5例λ链）。只有少数（6例）为T细胞谱系，所有病例对UCHL 1、MT 1和DFT 1均呈阳性反应。在6例T细胞淋巴瘤中，检测到1例Ber-H 2阳性间变性大细胞淋巴瘤。这种病例在原发性口腔结外非霍奇金淋巴瘤中首次被记录。5例可明确归为组织细胞系统。这些病例对组织蛋白酶D呈阳性，而识别Ia（HLA-DR）抗原的KP 1 LN 3在高级别B细胞淋巴瘤、T细胞淋巴瘤和真性组织细胞淋巴瘤中最常呈阳性。
