Ruco L P, Pomponi D, Pigott R, Stoppacciaro A, Monardo F, Uccini S, Boraschi D, Tagliabue A, Santoni A, Dejana E
Department of Biopathology, University La Sapienza, Rome, Italy.
Am J Pathol. 1990 Nov;137(5):1163-71.
Cryostat sections of 58 lymph nodes were immunostained with a polyclonal rabbit serum against IL-1 alpha, and with monoclonal antibodies directed to IL-1 alpha (Vmp18), IL-1 beta (Vhp20 and BRhC3), and tumor necrosis factor alpha (TNF alpha) (B154.7). Furthermore the presence of cytokine-containing cells was correlated with the expression of endothelial leukocyte adhesion molecule (ELAM-1; 29F2) and of human leukocyte antigen (HLA-DR) (OKIa-1) by endothelial cells. Cells containing IL-1 and/or TNF alpha were detected mainly in pathologic conditions characterized by reactive or neoplastic expansion of the lymph node paracortex. Cells positive for IL-1 were detected in 16 of 21 cases of Hodgkin's disease, in 4 of 4 cases of T-NHL, and in 5 cases of diffuse or mixed lymphadenitis. Interleukin-1 alpha was detected in macrophages, interdigitating reticulum cells (IDRCs), endothelial cells, and neoplastic Hodgkin's and Reed-Sternberg (H-RS) cells. Cells positive for IL-1 beta were much fewer and consisted mainly of macrophages. Hodgkin's Reed-Sternberg cells were negative for IL-1 beta even after in vitro stimulation with bacterial endotoxin. Tumor necrosis factor alpha (TNF alpha) was present in macrophages and H-RS cells. Endothelial leukocyte adhesion molecule-1 expression by endothelial venules was detected in 17 of 20 cases of Hodgkin's disease, in 2 of 4 cases of T-NHL, and in 5 of 5 cases of diffuse lymphadenitis. In these pathologic conditions, HLA-DR antigens also were expressed frequently by endothelial cells. Cytokine-containing cells and ELAM-1-positive high endothelial venules (HEV) were extremely rare in lymph nodes involved by follicular lymphadenitis (12 cases) or B-NHL (16 cases). In cases of reactive or neoplastic B-cell proliferations, HLA-DR-positive HEVs still were present often. Our results indicate that IL-1/TNF alpha production at tissue level is often associated with ELAM-1 expression by HEVs, but is less well correlated with expression of HLA-DR antigens by endothelial cells.
用抗IL-1α的兔多克隆血清以及针对IL-1α（Vmp18）、IL-1β（Vhp20和BRhC3）和肿瘤坏死因子α（TNFα）（B154.7）的单克隆抗体对58个淋巴结的低温恒温器切片进行免疫染色。此外，含细胞因子的细胞的存在与内皮细胞白细胞黏附分子（ELAM-1；29F2）和人类白细胞抗原（HLA-DR）（OKIa-1）的表达相关。含IL-1和/或TNFα的细胞主要在以淋巴结副皮质反应性或肿瘤性扩张为特征的病理情况下被检测到。在21例霍奇金病中的16例、4例T-NHL中的4例以及5例弥漫性或混合性淋巴结炎中检测到IL-1阳性细胞。在巨噬细胞、交错突网状细胞（IDRCs）、内皮细胞以及肿瘤性霍奇金和里德-斯腾伯格（H-RS）细胞中检测到白细胞介素-1α。IL-1β阳性细胞要少得多，主要由巨噬细胞组成。即使在体外经细菌内毒素刺激后，霍奇金里德-斯腾伯格细胞对IL-1β仍为阴性。肿瘤坏死因子α（TNFα）存在于巨噬细胞和H-RS细胞中。在20例霍奇金病中的17例、4例T-NHL中的2例以及5例弥漫性淋巴结炎中的5例中检测到内皮微静脉的内皮细胞白细胞黏附分子-1表达。在这些病理情况下，内皮细胞也经常表达HLA-DR抗原。在滤泡性淋巴结炎（12例）或B-NHL（16例）累及的淋巴结中，含细胞因子的细胞和ELAM-1阳性的高内皮微静脉（HEV）极为罕见。在反应性或肿瘤性B细胞增殖的病例中，HLA-DR阳性的HEV仍然经常存在。我们的结果表明，组织水平的IL-1/TNFα产生通常与HEV的ELAM-1表达相关，但与内皮细胞HLA-DR抗原的表达相关性较差。
