Department of Immunology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Reprod Immunol. 2012 Jun;94(2):216-21. doi: 10.1016/j.jri.2012.02.006. Epub 2012 Apr 12.
Preeclampsia (PE) is a pregnancy associated disorder characterized by hypertension and proteinuria, which causes neonatal and maternal morbidity and mortality. The Th1/Th2 cytokine paradigm of the immune adaptation in pregnancy is now expanded to include Th1/Th2/Th17 and regulatory T (Treg) cells. Among cytokines, TGFβ1 has properties that justify evaluation of its role in PE etiopathology. In this investigation the polymorphisms of the TGFβ1 gene at promoter region, positions -800G→A and -509C→T, were studied in 142 PE and 140 normal pregnant female subjects using PCR-RFLP. Additionally, serum TGFβ1 was determined by ELISA. At position -800G→A genotypes and allele frequencies showed no significant differences between PE patients (GG 73.9%; GA 21.1%; AA 4.93%) and normal control (GG 70%; GA 28.6%; AA 1.4%) women. However the AA genotype at this position was more frequent in PE patients than in the control group. At -509C→T position, genotypes and allele frequencies showed no significant differences between PE patients and control individuals. The CC genotype at -509C→T position was more prevalent in PE patients than in the control group. The mean serum TGFβ1 level was significantly higher (62.14 ng/ml) in PE patients compared with pregnant and non-pregnant control groups (and 47.01 and 40.68 ng/ml, respectively). In conclusion, the promoter region polymorphisms of TGFβ1 may not be associated with PE, but serum levels of this cytokine may contribute to the etiopathology of PE.
子痫前期（PE）是一种与妊娠相关的疾病，其特征为高血压和蛋白尿，可导致新生儿和产妇发病率和死亡率升高。妊娠期间免疫适应性的 Th1/Th2 细胞因子范式现已扩展到包括 Th1/Th2/Th17 和调节性 T（Treg）细胞。在细胞因子中，TGFβ1 具有使其在 PE 发病机制中作用合理化评估的特性。在本研究中，通过 PCR-RFLP 技术研究了 TGFβ1 基因启动子区域的 -800G→A 和 -509C→T 位置的多态性，共纳入 142 例 PE 患者和 140 例正常妊娠女性。此外，通过 ELISA 测定血清 TGFβ1。在位置 -800G→A 中，基因型和等位基因频率在 PE 患者（GG 73.9%；GA 21.1%；AA 4.93%）和正常对照组（GG 70%；GA 28.6%；AA 1.4%）之间没有显著差异。然而，该位置的 AA 基因型在 PE 患者中比对照组更常见。在 -509C→T 位置，基因型和等位基因频率在 PE 患者和对照组之间没有显著差异。-509C→T 位置的 CC 基因型在 PE 患者中比对照组更为常见。PE 患者的平均血清 TGFβ1 水平（62.14ng/ml）明显高于妊娠和非妊娠对照组（分别为 47.01ng/ml 和 40.68ng/ml）。总之，TGFβ1 启动子区域多态性可能与 PE 无关，但该细胞因子的血清水平可能有助于 PE 的发病机制。
