用大孔树脂吸附色谱法从泡盛曲霉发酵液中分离出布雷菲德菌素 A。
Isolation of brefeldin A from Eupenicillium brefeldianum broth using macroporous resin adsorption chromatography.
机构信息
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 May 1;895-896:146-53. doi: 10.1016/j.jchromb.2012.03.033. Epub 2012 Apr 1.
Brefeldin A (BFA) is a macrolide lactone antibiotic, possessing antitumor, antiviral, antifungal activities. In this work, a separation strategy involving one-step macroporous resin adsorption chromatography combined with crystallization was established for BFA purification from Eupenicillium brefeldianum CCTCC M 208113 fermentation broth. Among six macroporous resin adsorbents tested, the non-polar resin HZ830 had the best adsorption and desorption performance. The static equilibrium adsorption data fitted well with the Freundlich equation, and the adsorption kinetic followed the pseudo-second order model. Through experimental optimization of column adsorption and desorption, BFA in purity of 90.4% (w/w), 92.1% (w/w) yield was obtained by a one-step macroporous resin adsorption chromatography, using a stepwise elution protocol. Furthermore, high purity (>99%, w/w) of BFA crystals were prepared from E. brefeldianum CCTCC M 208113 fermentation broth in an overall recovery of 67.0% (w/w), using a combination of adsorption chromatography packed with non-polar macroporous adsorbent HZ830 and crystallization in acetone.
布雷菲德菌素 A（BFA）是一种大环内酯类抗生素，具有抗肿瘤、抗病毒、抗真菌活性。本工作建立了从青霉菌 CCTCC M 208113 发酵液中一步大孔树脂吸附色谱结合结晶法分离纯化 BFA 的工艺。在考察的六种大孔树脂中，非极性树脂 HZ830 对 BFA 的吸附和解吸性能最好。静态吸附平衡数据符合 Freundlich 方程，吸附动力学符合拟二级动力学模型。通过对柱吸附和解吸的实验优化，采用逐步洗脱方案，通过一步大孔树脂吸附色谱可得到纯度为 90.4%（w/w）、收率为 92.1%（w/w）的 BFA。进一步地，采用非极性大孔吸附树脂 HZ830 填充的吸附色谱和丙酮结晶相结合的方法，从青霉菌 CCTCC M 208113 发酵液中制备得到纯度大于 99%（w/w）的 BFA 晶体，总收率为 67.0%（w/w）。
Zotero 插件