Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, No. 18 Tianshui Middle Road, Lanzhou 730000, China.
J Agric Food Chem. 2011 Sep 14;59(17):9629-36. doi: 10.1021/jf2020232. Epub 2011 Aug 9.
Preparative separation and purification of rebaudioside A from steviol glycosides using mixed-mode macroporous adsorption resins (MARs) were systematically investigated. Mixed-mode MARs were prepared by a physical blending method. By evaluation of the adsorption/desorption ratio and adsorption/desorption capacity of mixed-mode MARs with different proportions toward RA and ST, the mixed-mode MAR 18 was chosen as the optimum strategy. On the basis of the static tests, it was found that the experimental data fitted best to the pseudosecond-order kinetics and Temkin-Pyzhev isotherm. Furthermore, the dynamic adsorption/desorption experiments were performed on the mini column packed with mixed-mode MAR 18. After one run treatment, the purity of rebaudioside A in purified product increased from 40.77 to 60.53%, with a yield rate of 38.73% (W/W), and that in residual product decreased from 40.77 to 36.17%, with a recovery yield of 57.61% (W/W). The total recovery yield reached 96.34% (W/W). The results showed that this method could be utilized in large-scale production of rebaudioside A from steviol glycosides in industry.
采用混合模式大孔吸附树脂（MARs）对甜菊糖苷中的莱鲍迪苷 A 进行了制备分离和纯化的系统研究。混合模式 MARs 通过物理共混法制备。通过评估不同比例的混合模式 MARs 对 RA 和 ST 的吸附/解吸比和吸附/解吸容量，选择混合模式 MAR 18 作为最佳策略。基于静态测试，发现实验数据最符合伪二级动力学和 Temkin-Pyzhev 等温线。此外，在填充有混合模式 MAR 18 的微型柱上进行了动态吸附/解吸实验。经过一次运行处理，纯化产物中莱鲍迪苷 A 的纯度从 40.77%提高到 60.53%，收率为 38.73%（W/W），而残留产物中莱鲍迪苷 A 的纯度从 40.77%降低到 36.17%，回收率为 57.61%（W/W）。总回收率达到 96.34%（W/W）。结果表明，该方法可用于工业上从甜菊糖苷中大规模生产莱鲍迪苷 A。
