Yao Ling-Yun, Zhu Yi-Xiang, Liu Chang-Qing, Jiao Rui-Hua, Lu Yan-Hua, Tan Ren-Xiang
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 May 1;989:122-8. doi: 10.1016/j.jchromb.2014.09.028. Epub 2014 Sep 28.
In this work, the separation and purification of fumigaclavine C (FC), an ergot alkaloid with strong anti-inflammatory activity from fermented mycelia of Aspergillus fumigatus was systematically evaluated. Among the eight tested resins, the non-polar resin D101 displayed the best adsorption and desorption based on of static adsorption and desorption tests. Adsorption isotherms were constructed on D101 resin and fitted well to the Freundlich model. Dynamic adsorption and desorption tests on a column packed with D101 resin have been investigated for optimization of chromatographic parameters. Under optimized conditions, the contents of FC increased from 7.32% (w/w) in the crude extract to 67.54% in the final product with a recovery yield of 90.35% (w/w) via one run. Furthermore, a lab scale-up separation was carried out, in which the FC content and recovery yield were 65.83% and 90.13%, respectively. These results demonstrated that this adsorption-desorption strategy by using D101 resin was simple and efficient, thus showing potential for large scale purification and preparation of FC in the future.
在本研究中，系统评估了从烟曲霉发酵菌丝体中分离纯化具有强抗炎活性的麦角生物碱烟曲霉棒曲霉素C（FC）的方法。在八种测试树脂中，基于静态吸附和解吸试验，非极性树脂D101表现出最佳的吸附和解吸性能。在D101树脂上构建了吸附等温线，并与Freundlich模型拟合良好。对填充D101树脂的色谱柱进行了动态吸附和解吸试验，以优化色谱参数。在优化条件下，通过一次运行，FC的含量从粗提取物中的7.32%（w/w）提高到最终产物中的67.54%，回收率为90.35%（w/w）。此外，还进行了实验室规模放大分离，其中FC含量和回收率分别为65.83%和90.13%。这些结果表明，使用D101树脂的这种吸附-解吸策略简单有效，因此在未来大规模纯化和制备FC方面具有潜力。
