Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, Tamilnadu, India.
Peptides. 2012 Jun;35(2):261-8. doi: 10.1016/j.peptides.2012.03.028. Epub 2012 Apr 3.
Peptide from croaker (Otolithes ruber) muscle protein hydrolysate was purified, characterized and evaluated for its in vitro and in vivo antioxidant activity. Results showed that purified peptide contained the amino acid sequence as Lys-Thr-Phe-Cys-Gly-Arg-His (861.6Da), which were expected to contribute to its antioxidant activities. This peptide efficiently quenched 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (84.5±1.2 and 62.4±2.9%), and successfully inhibits the lipid peroxidation and DNA damage and proven to be a potent antioxidant at different in vitro systems. It also improved the endogenous cellular antioxidant enzymes in Wistar rat by increasing the activities of catalase (CAT), glutathione-S-transferase (GST) and superoxide dismutase (SOD) after supplementation of the peptide (283.6±7.25, 4.3±0.78 and 28.42±1.97) compared to the negative control (196.4±5.65, 1.3±0.45 and 15.1±0.35). Therefore, croaker muscle peptide can increase an endurance capacity and facilitate recovery from oxidative stress.
从石首鱼肌肉蛋白水解物中纯化、鉴定并评价了其肽的体外和体内抗氧化活性。结果表明，纯化的肽含有赖氨酸-苏氨酸-苯丙氨酸-半胱氨酸-甘氨酸-精氨酸-组氨酸（861.6Da）序列，这有望有助于其抗氧化活性。该肽能有效猝灭 1,1-二苯基-2-苦基肼基（DPPH）和羟基自由基（84.5±1.2 和 62.4±2.9%），成功抑制脂质过氧化和 DNA 损伤，并在不同的体外系统中被证明是一种有效的抗氧化剂。它还通过增加补充肽后 Wistar 大鼠的内源性细胞抗氧化酶的活性（CAT、GST 和 SOD 的活性分别为 283.6±7.25、4.3±0.78 和 28.42±1.97）来提高其抗氧化能力，与阴性对照组（196.4±5.65、1.3±0.45 和 15.1±0.35）相比。因此，石首鱼肌肉肽可以提高耐力并促进氧化应激后的恢复。
