Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology-AIST, Ibaraki 305-8569, Japan.
Regul Toxicol Pharmacol. 2012 Jul;63(2):188-95. doi: 10.1016/j.yrtph.2012.03.014. Epub 2012 Apr 5.
The genotoxic potential of two products of multi-walled carbon nanotubes (coded as N-MWCNTs, diameter of 44 nm/BET surface area of 69 m²/g and MWNT-7, diameter of 70 nm/BET surface area of 23 m²/g) was evaluated using a battery of genotoxicity assays, comprising a bacterial reverse mutation test, an in vitro mammalian chromosomal aberration test, and a mammalian erythrocytes micronucleus test. Neither type exerted mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, and TA1537, or in Escherichia coli WP2uvrA, in the absence or presence of metabolic activation. The products of MWCNTs did not increase the number of structural chromosomal aberrations either, regardless of metabolic activation, though they increased the number of numerical chromosomal aberrations, one slightly and the other distinctly, in the absence of metabolic activation. In ICR mice, the two products did not affect the proportion of immature erythrocytes, the total proportion of erythrocytes, or the number of micronuclei in immature erythrocytes.
采用包括细菌回复突变试验、体外哺乳动物染色体畸变试验和哺乳动物红细胞微核试验在内的一系列遗传毒性试验，评估了两种多壁碳纳米管产物（分别编号为 N-MWCNTs，直径 44nm/比表面积 69m²/g 和 MWNT-7，直径 70nm/比表面积 23m²/g）的遗传毒性潜力。这两种产物在缺乏或存在代谢激活的情况下，均未使鼠伤寒沙门氏菌 TA98、TA100、TA1535 和 TA1537 ，或大肠杆菌 WP2uvrA 发生致突变性。无论是否存在代谢激活，MWCNTs 产物均未增加结构染色体畸变的数量，但在缺乏代谢激活的情况下，它们分别轻微和明显地增加了染色体数目畸变的数量。在 ICR 小鼠中，这两种产物不影响未成熟红细胞的比例、总红细胞比例或未成熟红细胞中的微核数量。
