Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
Regul Toxicol Pharmacol. 2011 Nov;61(2):192-8. doi: 10.1016/j.yrtph.2011.07.008. Epub 2011 Jul 29.
The genotoxic potential of a high purity sample of single-wall carbon nanotubes (SWCNTs) was evaluated using a battery of in vitro and in vivo genotoxicity assays. These comprised a bacterial reverse mutation test (Ames test), an in vitro chromosomal aberration test, and an in vivo mouse bone marrow micronucleus test. The SWCNTs exerted no genotoxicity in Salmonella typhimurium TA97, TA98, TA100, and TA1535, or in Escherichia coli WP2 uvrA/pKM101, whether in the absence or presence of metabolic activation and at concentrations of 12.5-500 μg/plate. In the chromosomal aberration test, at 300-1000 μg/mL, the SWCNTs did not increase the number of structural or numerical chromosomal aberrations, whether the test was conducted with or without metabolic activation. In the in vivo bone marrow micronucleus test, doses of 60 mg/kg and 200mg/kg SWCNTs did not affect the proportions of immature and total erythrocytes, nor did it increase the number of micronuclei in the immature erythrocytes of mice. The results of these studies show that the high purity and well-dispersed sample of SWCNTs are not genotoxic under the conditions of the in vitro bacterial reverse mutation assay, chromosomal aberration assay, or in vivo bone marrow micronucleus test, and thus appear not to pose a genotoxic risk to human health in vivo.
使用一系列体外和体内遗传毒性检测方法评估了高纯度单壁碳纳米管（SWCNTs）的遗传毒性潜力。这些方法包括细菌回复突变试验（Ames 试验）、体外染色体畸变试验和体内小鼠骨髓微核试验。无论是否存在代谢活化，SWCNTs 在浓度为 12.5-500μg/平板时，对鼠伤寒沙门氏菌 TA97、TA98、TA100 和 TA1535 或大肠杆菌 WP2 uvrA/pKM101 均未表现出遗传毒性。在染色体畸变试验中，SWCNTs 在 300-1000μg/mL 时，无论是否进行代谢活化，均未增加结构或数量染色体畸变的数量。在体内骨髓微核试验中，SWCNTs 的剂量为 60mg/kg 和 200mg/kg 时，不会影响未成熟和总红细胞的比例，也不会增加小鼠未成熟红细胞中的微核数量。这些研究的结果表明，在体外细菌回复突变试验、染色体畸变试验或体内骨髓微核试验条件下，高纯度和良好分散的 SWCNTs 样本不具有遗传毒性，因此在体内似乎不会对人类健康造成遗传毒性风险。
