Elk J R, Adair T, Drake R E, Gabel J C
Center for Microvascular and Lymphatic Studies, University of Texas Medical School, Houston.
Lymphology. 1990 Sep;23(3):145-8.
Increases in diaphragmatic lymph vessel flow (Qdi) may be important in preventing ascites because diaphragmatic lymph vessels drain the peritoneal space. However, lymphatic vessel function may be depressed in anesthetized, open chested animals. To test this hypothesis, we cannulated diaphragmatic lymph vessels in five sheep which were anesthetized with 1-2% halothane. We performed a thoracotomy and cannulated a diaphragmatic lymph vessel in each sheep. Then we infused 0.75-1.0 micrograms/kg of E. coli endotoxin intravenously and we measured Qdi and the lymph protein concentration for 2-4 hrs. The data were compared to previously reported data for five unanesthetized sheep (J. Appl. Physiol. 62:706-710, 1987). At baseline Qdi = 0.8 +/- 0.7 (SD) in the anesthetized sheep and 1.0 +/- 0.8 ml/hr in the unanesthetized sheep. After endotoxin, Qdi increased to 4.5 +/- 3.1 ml/hr in the unanesthetized sheep (p less than 0.05) but Qdi did not change in the anesthetized sheep. However, the lymph protein concentration increased similarly in each group, indicating that endotoxin caused the same degree of injury in each group. Our results indicate that diaphragmatic lymph vessel function is depressed in anesthetized, open chested sheep.
膈淋巴管流量（Qdi）的增加对于预防腹水可能很重要，因为膈淋巴管引流腹膜腔。然而，在麻醉的开胸动物中，淋巴管功能可能会受到抑制。为了验证这一假设，我们在五只使用1-2%氟烷麻醉的绵羊身上插管膈淋巴管。我们进行了开胸手术，并在每只绵羊身上插管一根膈淋巴管。然后我们静脉注射0.75-1.0微克/千克的大肠杆菌内毒素，并测量Qdi和淋巴蛋白浓度2-4小时。将这些数据与之前报道的五只未麻醉绵羊的数据（《应用生理学杂志》62:706-710，1987年）进行比较。在基线时，麻醉绵羊的Qdi = 0.8 +/- 0.7（标准差），未麻醉绵羊的Qdi为1.0 +/- 0.8毫升/小时。注射内毒素后，未麻醉绵羊的Qdi增加到4.5 +/- 3.1毫升/小时（p < 0.05），但麻醉绵羊的Qdi没有变化。然而，每组的淋巴蛋白浓度升高相似，表明内毒素在每组中造成的损伤程度相同。我们的结果表明，在麻醉的开胸绵羊中，膈淋巴管功能受到抑制。
