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使用免疫成像扫描探针显微镜对聚（酰胺胺）PAMAM树枝状大分子进行低水平检测。

Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy.

作者信息

Cason Chevelle A, Fabré Thomas A, Buhrlage Andrew, Haik Kristi L, Bullen Heather A

机构信息

Department of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USA.

出版信息

Int J Anal Chem. 2012;2012:341260. doi: 10.1155/2012/341260. Epub 2012 Feb 8.

DOI:10.1155/2012/341260
PMID:22505915
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3296299/
Abstract

Immunoimaging scanning probe microscopy was utilized for the low-level detection and quantification of biotinylated G4 poly(amidoamine) PAMAM dendrimers. Results were compared to those of high-performance liquid chromatography (HPLC) and found to provide a vastly improved analytical method for the low-level detection of dendrimers, improving the limit of detection by a factor of 1000 (LOD = 2.5 × 10(-13) moles). The biorecognition method is reproducible and shows high specificity and good accuracy. In addition, the capture assay platform shows a promising approach to patterning dendrimers for nanotechnology applications.

摘要

免疫成像扫描探针显微镜用于生物素化的G4聚（酰胺胺）PAMAM树枝状大分子的低水平检测和定量。将结果与高效液相色谱（HPLC）的结果进行比较，发现它为树枝状大分子的低水平检测提供了一种大大改进的分析方法，检测限提高了1000倍（LOD = 2.5×10⁻¹³摩尔）。这种生物识别方法具有可重复性，表现出高特异性和良好的准确性。此外，捕获分析平台为纳米技术应用中树枝状大分子的图案化提供了一种有前景的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/0b1d76950f3f/IJAC2012-341260.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/1f209e84db3b/IJAC2012-341260.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/b5679d068c9d/IJAC2012-341260.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/c7c30c230744/IJAC2012-341260.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/ecbe2b4fecd6/IJAC2012-341260.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/0b1d76950f3f/IJAC2012-341260.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/1f209e84db3b/IJAC2012-341260.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/b5679d068c9d/IJAC2012-341260.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/c7c30c230744/IJAC2012-341260.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/ecbe2b4fecd6/IJAC2012-341260.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08a4/3296299/0b1d76950f3f/IJAC2012-341260.005.jpg

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