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利用自组装单分子层/还原氧化石墨烯-PAMAM 树枝状大分子薄膜-SPR 光学传感器灵敏检测登革热病毒 2 型 E 蛋白信号。

Sensitive Detection of Dengue Virus Type 2 E-Proteins Signals Using Self-Assembled Monolayers/Reduced Graphene Oxide-PAMAM Dendrimer Thin Film-SPR Optical Sensor.

机构信息

Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

出版信息

Sci Rep. 2020 Feb 11;10(1):2374. doi: 10.1038/s41598-020-59388-3.

DOI:10.1038/s41598-020-59388-3
PMID:32047209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7012912/
Abstract

In this work, sensitive detection of dengue virus type 2 E-proteins (DENV-2 E-proteins) was performed in the range of 0.08 pM to 0.5 pM. The successful DENV detection at very low concentration is a matter of concern for targeting the early detection after the onset of dengue symptoms. Here, we developed a SPR sensor based on self-assembled monolayer/reduced graphene oxide-polyamidoamine dendrimer (SAM/NHrGO/PAMAM) thin film to detect DENV-2 E-proteins. Surface characterizations involving X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) confirms the incorporation of NHrGO-PAMAM nanoparticles in the prepared sensor films. The specificity, sensitivity, binding affinity, and selectivity of the SPR sensor were then evaluated. Results indicated that the variation of the sensing layer due to different spin speed, time incubation, and concentration provided a better interaction between the analyte and sensing layer. The linear dependence of the SPR sensor showed good linearity (R = 0.92) with the lowest detection of 0.08 pM DENV-2 E-proteins. By using the Langmuir model, the equilibrium association constant was obtained at very high value of 6.6844 TM (R = 0.99). High selectivity of the SPR sensor towards DENV-2 E-proteins was achieved in the presence of other competitors.

摘要

在这项工作中，对登革热病毒 2 型 E 蛋白（DENV-2 E-proteins）进行了在 0.08 pM 到 0.5 pM 范围内的灵敏检测。在非常低的浓度下成功检测到 DENV 是一个值得关注的问题，因为这是针对登革热症状发作后早期检测的目标。在这里，我们开发了一种基于自组装单层/还原氧化石墨烯-聚酰胺胺树枝状大分子（SAM/NHrGO/PAMAM）薄膜的 SPR 传感器来检测 DENV-2 E 蛋白。涉及 X 射线衍射（XRD）和傅里叶变换红外光谱（FTIR）的表面特性证实了 NHrGO-PAMAM 纳米粒子在制备的传感器薄膜中的掺入。然后评估了 SPR 传感器的特异性、灵敏度、结合亲和力和选择性。结果表明，由于不同的旋转速度、孵育时间和浓度，传感层的变化提供了分析物和传感层之间更好的相互作用。SPR 传感器的线性依赖性显示出与最低检测限为 0.08 pM 的 DENV-2 E 蛋白的良好线性（R = 0.92）。通过使用 Langmuir 模型，获得了非常高的值 6.6844 TM（R = 0.99）的平衡结合常数。SPR 传感器对 DENV-2 E 蛋白具有高选择性，即使存在其他竞争物也是如此。

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