Sukhorukova E G, Alekseeva O S, Kirik O V, Grudinina N A, Korzhevskiĭ D E
Morfologiia. 2011;140(6):32-5.
A study of structural and functional organization of the boundaries separating CNS compartments is a fundamental task of neurobiology. Taking into account the contradictory data on the structure of superficial layers of mammalian neocortex, it is pertinent to study structural and cytochemical organization of astrocytes--the main components of the brain barrier system in animals that are often used for experimental modeling of brain diseases and injuries. The aim of the present work was to study the structural organization of layer I astrocytes of rat neocortex. Astrocytes were demonstrated immunocytochemically using anti-GFAP, anti-vimentin and anti-nestin antibodies using light and confocal laser microscopy. The results of the study demonstrated that the superficial glial limiting membrane had significant structural differences in different cortical regions. Astrocytes in layer I of rat neocortex were different from typical protoplasmic astrocytes, common to gray matter The regional peculiar features of superficial glial limiting membrane organization that were found in this study, are probably determined by the differences in functional characteristics of CSF-encephalic barrier in the specific regions of the brain.
研究分隔中枢神经系统各部分的边界的结构和功能组织是神经生物学的一项基本任务。考虑到关于哺乳动物新皮质表层结构的相互矛盾的数据，研究星形胶质细胞的结构和细胞化学组织是有意义的，星形胶质细胞是动物脑屏障系统的主要组成部分，常用于脑部疾病和损伤的实验建模。本研究的目的是研究大鼠新皮质I层星形胶质细胞的结构组织。使用抗GFAP、抗波形蛋白和抗巢蛋白抗体，通过光镜和共聚焦激光显微镜对星形胶质细胞进行免疫细胞化学检测。研究结果表明，表面神经胶质界膜在不同皮质区域存在显著的结构差异。大鼠新皮质I层的星形胶质细胞不同于灰质中常见的典型原浆性星形胶质细胞。本研究中发现的表面神经胶质界膜组织的区域特征可能由脑特定区域脑脊液-脑屏障功能特征的差异所决定。
