University of Birmingham, Birmingham, UK.
Anaesthesia. 2012 Aug;67(8):870-4. doi: 10.1111/j.1365-2044.2012.07151.x. Epub 2012 Apr 16.
We report laboratory and clinical evaluations of a blood propofol concentration analyser. Laboratory experiments used volunteer blood spiked with known propofol concentrations over the clinically relevant concentrations from 0.5 to 16 μg.ml(-1) to assess linearity and the influence of haematocrit and concurrent drug administration. Analyser concentrations demonstrated excellent linearity (R(2) = 0.999). Blood spiked with commonly used drugs showed no significant variation compared to unspiked controls. Propofol measurements were largely independent of haemoglobin concentration. A 6% decay in propofol concentration was observed at the highest prepared concentration. Clinical performance of the analyser was assessed using 80 arterial blood samples from 72 patients receiving propofol infusions during cardiac surgery. Samples were processed using the propofol analyser, and high performance liquid chromatography (HPLC) used as a gold-standard comparator. These data demonstrated excellent agreement between the propofol analyser and HPLC with a bias of 0.13 μg.ml(-1) and precision of -0.16 to 0.42 μg.ml(-1).
我们报告了一种血液异丙酚浓度分析仪的实验室和临床评估。实验室实验使用志愿者血液，在 0.5 至 16μg.ml（-1）的临床相关浓度范围内加入已知浓度的异丙酚，以评估线性度和血细胞比容以及同时给药的影响。分析仪浓度表现出极好的线性度（R（2）= 0.999）。与未加样对照相比，加入常用药物的血液没有显示出明显的变化。与血红蛋白浓度相比，异丙酚的测量结果在很大程度上是独立的。在最高制备浓度下，观察到异丙酚浓度下降 6%。使用在心脏手术期间接受异丙酚输注的 72 名患者的 80 份动脉血样评估分析仪的临床性能。使用异丙酚分析仪处理样品，并使用高效液相色谱（HPLC）作为金标准比较器。这些数据表明，异丙酚分析仪与 HPLC 之间具有极好的一致性，偏差为 0.13μg.ml（-1），精度为-0.16 至 0.42μg.ml（-1）。
