[痘苗病毒基因组的分子生物学研究。I. 痘苗病毒DNA片段在细菌载体中的克隆]
[Molecular-biological study of vaccinia virus genome. I. Cloning of vaccinia virus DNA fragments in bacterial vectors].
作者信息
Riazankina O I, Totmenin A V, Shchelkunov S N, Malygin E G
出版信息
Mol Biol (Mosk). 1990 Jul-Aug;24(4):962-7.
PMID:2250684
Abstract
The HindIII DNA fragments of vaccinia virus strain L-IVP were cloned in pBR322 bacterial plasmid. A hybrid plasmids collection of pVHn series contains all fragments of virus genome except terminal HindIII-B and HindIII-G, and also a large HindIII-A. The latter was cloned in cosmid pHC79. The obtained collection of hybrid DNA molecules allows to carry out a wide range of molecular biological experiments on the vaccinia virus genome.
摘要
痘苗病毒L-IVP株的HindIII DNA片段被克隆到pBR322细菌质粒中。pVHn系列的杂交质粒集合包含病毒基因组除末端HindIII-B和HindIII-G之外的所有片段，以及一个大的HindIII-A片段。后者被克隆到黏粒pHC79中。所获得的杂交DNA分子集合使得能够对痘苗病毒基因组进行广泛的分子生物学实验。
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