Netesova N A, Muravlev A I, Chikaev N A, Malygin E G
Mol Biol (Mosk). 1991 Nov-Dec;25(6):1526-32.
The I5 gene from the HindIII-I-fragment of the vaccinia virus strain L-IVP DNA was cloned into bacterial vector pUC19. The monospecific antiserum to the expression product of this gene in E. coli was obtained. This antiserum was demonstrated to co-precipitate the virion protein p90. The vaccinia virus strain L-LVP DNA was shown to have only one ORF coding the p90 protein instead of two ORF H5 and H4 as known for vaccinia virus strain WR. This protein is associated with the core of vaccinia virion, but some of its antigenic determinants are exposed on the surface of the viral particles.
将痘苗病毒株L-IVP DNA的HindIII-I片段中的I5基因克隆到细菌载体pUC19中。获得了针对该基因在大肠杆菌中表达产物的单特异性抗血清。该抗血清被证明能共沉淀病毒粒子蛋白p90。结果表明，痘苗病毒株L-LVP DNA只有一个编码p90蛋白的开放阅读框，而不像痘苗病毒株WR那样有两个开放阅读框H5和H4。这种蛋白与痘苗病毒粒子的核心相关，但它的一些抗原决定簇暴露在病毒颗粒表面。
