Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, PR China.
J Craniomaxillofac Surg. 2013 Sep;41(6):476-86. doi: 10.1016/j.jcms.2012.03.011. Epub 2012 Apr 15.
The purpose of this paper is to summarize our experiences in creating an animal model of TMJ bony ankylosis based on 2 sequential experiments.
Two sequential experiments were performed with the aim of creating a model of TMJ bony ankylosis. Seven growing sheep were used in the first experiment, in which 1 was served as a control animal. Condylar fracture with disc preservation was performed on the control side. On the contralateral side, condylar fracture, excision of the lateral 2/3 disc and injury to the glenoid fossa were performed to induce bony ankylosis. Three animals were sacrificed respectively at 3 and 6 months after surgery. In the second experiment, 7 growing sheep were used. The only difference of modeling between the 2 experiments was that more serious injury to the glenoid fossa was made in the ankylosis-induced side in experiment 2. Three, 2, and 2 animals were sacrificed respectively at 1, 3, and 6 months postoperatively. The TMJ complexes were examined by computed tomography (CT) and histological evaluation.
In experiment 1, only fibrous ankylosis was observed in the ankylosis-induced side both at 3 and 6 months postoperatively. In experiment 2, CT and histological evaluation showed that the outcomes of the ankylosis-induced side were fibrous-bony ankylosis, fibrous-bony ankylosis, and bony ankylosis respectively at 1, 3, and 6 months after surgery.
Through summarizing the differences of the modeling and the different outcomes in the 2 experiments, we concluded that severe damage to the glenoid fossa played an important role in the development of TMJ bony ankylosis.
本文旨在总结我们基于 2 项连续实验创建颞下颌关节骨强直动物模型的经验。
进行了 2 项连续实验，旨在创建颞下颌关节骨强直模型。第 1 项实验使用了 7 只生长中的绵羊，其中 1 只为对照动物。在对照侧进行髁突骨折伴盘保留术。在对侧，行髁突骨折、外侧 2/3 盘切除和关节窝损伤以诱导骨强直。术后 3 个月和 6 个月分别处死 3 只动物。第 2 项实验使用了 7 只生长中的绵羊。2 项实验建模的唯一区别是在第 2 项实验中，在强直诱导侧对关节窝造成更严重的损伤。术后 1、3 和 6 个月分别处死 3、2 和 2 只动物。通过 CT 和组织学评估检查 TMJ 复合体。
在第 1 项实验中，术后 3 个月和 6 个月，强直诱导侧仅观察到纤维性强直。在第 2 项实验中，CT 和组织学评估显示，强直诱导侧术后 1、3 和 6 个月的结果分别为纤维骨性强直、纤维骨性强直和骨性强直。
通过总结 2 项实验建模的差异和不同结果，我们得出结论，关节窝的严重损伤在颞下颌关节骨性强直的发展中起着重要作用。
