Yan Ying-Bin, Li Jiang-Ming, Xiao E, An Jin-Gang, Gan Ye-Hua, Zhang Yi
Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China; Department of Oral and Maxillofacial Surgery, Tianjin Stomatological Hospital, 75 Dagu Road, Heping District, Tianjin 300041, PR China.
Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
J Craniomaxillofac Surg. 2014 Mar;42(2):e23-8. doi: 10.1016/j.jcms.2013.04.008. Epub 2013 May 24.
The purpose of the study was to preliminarily explore the differential expressions of a series of genes regulating bone formation in temporomandibular joint (TMJ) fibrous ankylosis, bony ankylosis and condylar fracture healing.
The cDNA from either the bony ankylosed callus or fracture callus of the 6 sheep, as described in the part I, were both used in the study. The differences of gene expressions between bony ankylosis and condylar fracture at 1, 3, and 6 months postoperatively were measured by real-time PCR, with 2 samples at each time point. In addition, another 2 sheep were added to have fibrous ankylosis induced on the right TMJ, and 1 sheep was sacrificed at 3 and 6 months after surgery, respectively. The differences of gene expressions between fibrous and bony ankylosis at 3 and 6 months postoperatively were measured by real-time PCR.
Bony ankylosis showed higher mRNA expression trends in Wnt2b, Wnt5a, β-Catenin, Lef1, CyclinD1, Runx2, Osterix, Sox9, Col10a1, Alp, Ocn, Bmp2, and Bmp7 compared to fibrous ankylosis, although no statistical analysis was performed due to the very small sample size. Whereas bony ankylosis showed a significant lower expression of Wnt5a, β-Catenin, Lef1, Runx2, Osterix, Sox9, Col10a1, Alp, Ocn and Bmp4 compared to condylar fracture at several time points (P < 0.05).
Our data provided a preliminary molecular evidence for the hypothesis that the development of traumatic TMJ bony ankylosis was the course of delayed bone healing or hypertrophic nonunion, and deserved to be further studied.
本研究旨在初步探讨一系列调控骨形成的基因在颞下颌关节（TMJ）纤维性强直、骨性强直及髁突骨折愈合中的差异表达。
本研究采用了第一部分所述的6只绵羊的骨性强直骨痂或骨折骨痂的cDNA。通过实时PCR检测术后1、3和6个月时骨性强直与髁突骨折之间的基因表达差异，每个时间点有2个样本。此外，另外添加2只绵羊，在其右侧TMJ诱导纤维性强直，分别在术后3个月和6个月处死1只绵羊。通过实时PCR检测术后3个月和6个月时纤维性强直与骨性强直之间的基因表达差异。
与纤维性强直相比，骨性强直在Wnt2b、Wnt5a、β-连环蛋白、Lef1、细胞周期蛋白D1、Runx2、Osterix、Sox9、Col10a1、碱性磷酸酶（Alp）、骨钙素（Ocn）、骨形态发生蛋白2（Bmp2）和骨形态发生蛋白7（Bmp7）中显示出更高的mRNA表达趋势，尽管由于样本量非常小未进行统计分析。然而，在几个时间点上，与髁突骨折相比，骨性强直的Wnt5a、β-连环蛋白、Lef1、Runx2、Osterix、Sox9、Col10a1、Alp、Ocn和骨形态发生蛋白4（Bmp4）表达显著降低（P<0.05）。
我们的数据为创伤性TMJ骨性强直的发生发展是延迟骨愈合或肥厚性骨不连过程这一假说提供了初步的分子证据，值得进一步研究。
