Chauvet J, Michel G, Rouillé Y, Chauvet M T, Acher R
Laboratory of Biological Chemistry, University of Paris VI 96, France.
Neuropeptides. 1990 Mar;15(3):123-7. doi: 10.1016/0143-4179(90)90142-l.
Xenopus laevis neurophysins have been purified from neurointermediate pituitaries through high-pressure reverse-phase liquid chromatography and their N-terminal amino acid sequences have been determined by microsequencing. Two types of neurophysins, corresponding to mammalian MSEL- and VLDV-neurophysins, have been distinguished. A strong homology exists between neurophysins of Xenopus (Pipidae), frog (Ranidae) and toad (Bufonidae). Xenopus MSEL-neurophysin, as frog MSEL-neurophysin, has a high molecular mass suggesting that the C-terminal domain of the vasotocin precursor is not processed in contrast to the two-step processing observed for mammalian vasopressin precursor.
Mammalian neurophysins are termed MSEL- and VLDV-neurophysins according to the nature of residues in positions 2, 3, 6 and 7 (one-letter symbols for amino acids).
非洲爪蟾神经垂体素已通过高压反相液相色谱法从神经中间叶垂体中纯化出来，其N端氨基酸序列已通过微量测序法确定。已区分出两种类型的神经垂体素，分别对应于哺乳动物的MSEL-神经垂体素和VLDV-神经垂体素。非洲爪蟾（负子蟾科）、青蛙（蛙科）和蟾蜍（蟾蜍科）的神经垂体素之间存在很强的同源性。与青蛙的MSEL-神经垂体素一样，非洲爪蟾的MSEL-神经垂体素具有高分子量，这表明与哺乳动物血管加压素前体的两步加工过程不同，血管紧张素原前体的C端结构域未被加工。
根据第2、3、6和7位残基的性质（氨基酸的单字母符号），哺乳动物神经垂体素被称为MSEL-神经垂体素和VLDV-神经垂体素。
