Chauvet J, Michel G, Chauvet M T, Acher R
Laboratory of Biological Chemistry, University of Paris VI, France.
FEBS Lett. 1988 Mar 28;230(1-2):77-80. doi: 10.1016/0014-5793(88)80645-8.
A 'big' frog (Rana esculenta) neurophysin, encompassing sequences homologous to mammalian MSEL-neurophysin and copeptin, has been passed through a trypsin-Sepharose column in order to compare its conformation with that of the two-domain intermediate precursor isolated from guinea pig. Whereas the polypeptide possesses 8 arginine residues, only two cleavages were observed located in a putative inter-domain sequence (at Arg-94 and Arg-114). Because free vasotocin has been isolated from the frog, it is assumed that pro-vasotocin has a three-domain conformation similar to that of pro-vasopressin but processing in amphibians involves only one step rather than two steps as in mammals.
一种“大型”青蛙（食用蛙）神经垂体激素运载蛋白，包含与哺乳动物MSEL-神经垂体激素运载蛋白和 copeptin 同源的序列，已通过胰蛋白酶-琼脂糖凝胶柱，以便将其构象与从豚鼠分离出的双结构域中间前体的构象进行比较。尽管该多肽含有8个精氨酸残基，但仅在一个假定的结构域间序列（位于精氨酸-94和精氨酸-114处）观察到两次切割。由于已从青蛙中分离出游离的血管催产素，因此推测血管催产素原具有与血管加压素原相似的三结构域构象，但两栖动物中的加工过程仅涉及一个步骤，而不像哺乳动物那样涉及两个步骤。
