Veterinary Public Health, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, South Korea.
Vaccine. 2012 Jun 6;30(26):3829-33. doi: 10.1016/j.vaccine.2012.03.087. Epub 2012 Apr 14.
In this study, a comparative evaluation of a novel live vaccine candidate expressing enterotoxigenic Escherichia coli (ETEC) fimbriae and a commercial ETEC vaccine was carried out in suckling to weaned piglets. The E. coli K88ab, K88ac, K99, FasA and F41 fimbrial genes were individually inserted into an expression/secretion plasmid, pBP244. These plasmids were subsequently transfected into attenuated Salmonella, which were used as the vaccine candidate. Eighteen pregnant sows and 107 of their piglets were used in this comparative study. All the vaccinated groups of sows and piglets exhibited significantly increased antibody levels relative to specific antigens when compared with those in the unimmunized control. The experimental piglets with the vaccine candidate did not experience diarrhea following challenge with the virulent ETEC strains. However, diarrhea was observed in 36.8% of the piglets in the group immunized with the commercial vaccine and in 50% of the control group after challenge with the ETEC strains. These findings indicate that immunization of sows with the candidate vaccine can effectively protect their young pigs against colibacillosis.
本研究对一种新型表达肠产毒性大肠杆菌（ETEC）菌毛的活疫苗候选物和一种商业 ETEC 疫苗进行了比较评估，在哺乳期和断奶仔猪中进行了研究。将大肠杆菌 K88ab、K88ac、K99、FasA 和 F41 菌毛基因分别插入表达/分泌质粒 pBP244 中。这些质粒随后被转染到减毒沙门氏菌中，作为疫苗候选物。在这项比较研究中，使用了 18 头怀孕母猪和 107 头仔猪。与未免疫对照组相比，所有接种疫苗的母猪和仔猪组的抗体水平均显著升高。与强毒 ETEC 菌株攻毒后，实验组仔猪未出现腹泻，但在接种商业疫苗的仔猪组中，有 36.8%出现腹泻，在对照组中，有 50%出现腹泻。这些发现表明，用候选疫苗免疫母猪可以有效保护其仔猪免受大肠杆菌病的侵害。
