Research and Development Department, Nippon Supplement, Inc., 19-19, Chayamachi, Kita-Ku, Osaka, 530-0013, Japan.
Phytother Res. 2012 Apr;26(4):475-81. doi: 10.1002/ptr.3602. Epub 2011 Aug 16.
The antiobesity effects of Chinese black tea (Pu-erh tea) and of gallic acid (GA) were investigated using in vitro and in vivo assays. Chinese black tea extract (BTE) and GA inhibited pancreatic lipase activity in a dose-dependent manner in vitro; the IC(inhibitory concentration)(50) values were 101.6 and 9.2 µg/mL, respectively. Black tea extract (50, 100 mg/kg body weight (b.w.)) and GA (15, 45 mg/kg b.w.) significantly suppressed the elevation of blood triglyceride after oral administration of a corn oil emulsion (8 mL oil/kg b.w.) to male ddY mice. Moreover, the antiobesity effects of BTE and GA were also evaluated in a mouse model of diet-induced obesity. Female ddY mice were divided into seven groups; normal diet (ND) group, high fat diet (HFD) group, BTE (0.2% and 0.6% of diets) groups, and GA (0.007%, 0.02% and 0.1% of diets) groups; the experimental groups were fed the test diets for 12 weeks. The BTE 0.6% and GA 0.1% groups showed significant suppression of weight gain. The weight of parametrial adipose tissue was strongly correlated with the body weight. These results suggest that GA contributes to the antiobesity effect of BTE as an active constituent by inhibiting pancreatic lipase activity.
采用体外和体内试验研究了中国黑茶（普洱茶）和没食子酸（GA）的抗肥胖作用。中国黑茶提取物（BTE）和 GA 呈剂量依赖性抑制胰脂肪酶活性；IC（50）值分别为 101.6 和 9.2 µg/mL。黑茶提取物（50、100 mg/kg 体重（b.w.））和 GA（15、45 mg/kg b.w.）显著抑制雄性 ddY 小鼠口服玉米油乳液（8 mL 油/kg b.w.）后血液甘油三酯的升高。此外，还在饮食诱导肥胖的小鼠模型中评估了 BTE 和 GA 的抗肥胖作用。将雌性 ddY 小鼠分为 7 组；正常饮食（ND）组、高脂肪饮食（HFD）组、BTE（饮食的 0.2%和 0.6%）组和 GA（饮食的 0.007%、0.02%和 0.1%）组；实验组喂食测试饮食 12 周。BTE 0.6%和 GA 0.1%组体重增加明显受到抑制。腹膜后脂肪组织的重量与体重强烈相关。这些结果表明，GA 通过抑制胰脂肪酶活性，作为一种活性成分，有助于 BTE 的抗肥胖作用。
