Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, South Korea.
J Periodontol. 2013 Jan;84(1):110-6. doi: 10.1902/jop.2012.110241. Epub 2012 Apr 17.
Previous studies showed that the use of a porous titanium membrane (TM) for exophytic bone regeneration does not effectively inhibit the infiltration of undesired tissue. Therefore, this study examines the effect of resorbable collagen membranes, such as cross-linked type I collagen membrane (BA) and double-layered porcine collagen membrane (BG), on the promotion of exophytic bone formation in guided bone regeneration when used in conjunction with a porous TM.
Thirty-six male New Zealand white rabbits were used in this study. Six rabbits were allotted to each test group. After decorticating the parietal bone, with or without filling the inner space with a freeze-dried cortical bone allograft (OG), the collagen membranes were fixed with metal pins. The experimental groups were divided into the following six groups: TM only, TM + OG, TM + BA, TM + BG, TM + OG + BA, and TM + OG + BG. The experimental animals were sacrificed at 8 and 16 weeks after surgery. Non-decalcified specimens were prepared and processed for histologic observations. The newly formed bone (percentage) was measured histomorphometrically.
BG combined with TM promoted new bone formation and maturation by inhibiting the infiltration of connective tissue. However, BA had no significant effect on new bone formation. The amount of new bone formation was higher at 16 weeks than at 8 weeks, but the difference was not significant. At 16 weeks, the best result for newly formed bone was with TM + OG + BG, with a significant difference from TM alone and TM + BA.
Regardless of the use of graft materials, BG combined with TM promoted more bone formation than BA combined with TM or TM alone. Thus, using a commercial collagen membrane to cover a TM can promote new exophytic bone formation.
先前的研究表明，使用多孔钛膜（TM）进行外生性骨再生并不能有效地抑制不需要的组织的渗透。因此，本研究探讨了可吸收胶原膜（如交联型 I 型胶原膜（BA）和双层猪胶原膜（BG））在与多孔 TM 结合使用时对促进引导骨再生中外生性骨形成的影响。
本研究使用 36 只雄性新西兰白兔。将 6 只兔子分配到每个测试组。在去皮质骨后，用或不用冻干皮质骨移植物（OG）填充内部空间，将胶原膜用金属钉固定。实验分组如下：TM 组、TM+OG 组、TM+BA 组、TM+BG 组、TM+OG+BA 组和 TM+OG+BG 组。手术后 8 周和 16 周处死实验动物。制备非脱钙标本并进行组织学观察。组织形态计量学测量新形成的骨（百分比）。
BG 与 TM 结合可通过抑制结缔组织的渗透来促进新骨的形成和成熟。然而，BA 对新骨形成没有显著影响。16 周时新骨形成量高于 8 周，但差异无统计学意义。在 16 周时，新骨形成量最好的是 TM+OG+BG 组，与 TM 组和 TM+BA 组相比有显著差异。
无论是否使用移植物材料，BG 与 TM 结合均比 BA 与 TM 结合或 TM 单独使用更能促进骨形成。因此，使用商业胶原膜覆盖 TM 可以促进新的外生性骨形成。
