Kim Su-Hwan, Kim Do-Young, Kim Kyoung-Hwa, Ku Young, Rhyu In-Chul, Lee Yong-Moo
Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
Clin Oral Implants Res. 2009 Oct;20(10):1124-32. doi: 10.1111/j.1600-0501.2009.01744.x. Epub 2009 Jun 7.
To analyze the outcomes of a single- and a double-layered collagen membrane on the efficacy of onlay block grafts in terms of bone resorption and augmentation.
A total of 36 New Zealand white rabbits were used in this study. Calvarial bone blocks were obtained from one side of the parietal bone and fixed on the contaralateral side. The onlay grafts were covered with either no (C group), one (M1 group), or two (M2 group) layers of a non-cross-linked collagen membrane (BioGide((R))). After 2, 4, and 6 months of healing, rabbits were sacrificed and explanted specimens were prepared for histologic and histomorphometric analysis. At each period, the overall pattern of graft bone resorption and membrane biodegradation were examined histologically, and the sustained external form of grafted bone (%) and the remaining mineralized bone volume (%) were measured histomorphometrically.
The M1 and M2 groups exhibited decreased bone resorption compared with the C group at all periods. The M2 group had less graft resorption and a higher bone density of the grafted bone than the M1 group. In the M1 group, the collagen membrane was degraded partially or completely by 4 months and was absent at 6 months. Conversely, for the M2 group, the membrane body was retained up to 6 months.
Our results demonstrated that the collagen membrane used here can reduce graft bone resorption. Furthermore, the double-layer technique using non-cross-linked collagen membranes (BioGide((R))) can enhance the efficacy of the onlay block bone graft technique in terms of both bone resorption and augmentation compared with a single-layer collagen membrane.
分析单层和双层胶原膜对覆盖式块状移植骨在骨吸收和骨增量方面疗效的影响。
本研究共使用36只新西兰白兔。从顶骨一侧获取颅骨块并固定于对侧。覆盖式移植骨分别不覆盖（C组）、覆盖一层（M1组）或两层（M2组）非交联胶原膜（BioGide®）。愈合2、4和6个月后，处死兔子，制备移植标本用于组织学和组织形态计量学分析。在每个时期，通过组织学检查移植骨吸收和膜生物降解的总体模式，并通过组织形态计量学测量移植骨的持续外形（%）和剩余矿化骨体积（%）。
在所有时期，M1组和M2组的骨吸收均低于C组。M2组的移植骨吸收少于M1组，且移植骨密度更高。在M1组，胶原膜在4个月时部分或完全降解，6个月时消失。相反，在M2组，膜体保留至6个月。
我们的结果表明，此处使用的胶原膜可减少移植骨吸收。此外，与单层胶原膜相比，使用非交联胶原膜（BioGide®）的双层技术在骨吸收和骨增量方面均可提高覆盖式块状骨移植技术的疗效。
