Terry Fox Laboratory, British Columbia Cancer Agency and the Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada.
Exp Hematol. 2012 Aug;40(8):601-11. doi: 10.1016/j.exphem.2012.04.001. Epub 2012 Apr 14.
Understanding the steps and cues that allow hematopoietic cells to be generated during development holds great clinical as well as biological interest. Analysis of these events in mice has provided many important insights into the processes involved, but features that might be unique to humans remain challenging to elucidate because they cannot be studied directly in vivo. Human embryonic stem or induced pluripotent stem cells offer attractive in vitro alternatives to analyze the process. Here we review recent efforts to develop defined and quantitative systems to address outstanding developmental questions against a background of what we know about the development of hematopoietic cells in the fetus and derived from mouse embryonic stem cells.
了解造血细胞在发育过程中产生的步骤和线索具有重要的临床和生物学意义。对这些事件在小鼠中的分析提供了许多重要的见解，但人类特有的特征仍然难以阐明，因为它们不能直接在体内进行研究。人类胚胎干细胞或诱导多能干细胞为分析这一过程提供了有吸引力的体外替代方法。在这里，我们回顾了最近在胎儿造血细胞发育和从小鼠胚胎干细胞中已知的背景下，为解决未解决的发育问题而开发明确和定量系统的努力。
