State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Phytother Res. 2013 Feb;27(2):186-91. doi: 10.1002/ptr.4698. Epub 2012 Apr 18.
The objective of this study was to evaluate the immunomodulatory effects of the purified Ganoderma atrum polysaccharide (PSG-1) on murine macrophage cell line RAW264.7. Phagocytotic assay by fluorescein isothiocyanate-dextran internalization showed that PSG-1 stimulated the phagocytosis of macrophages. G. atrum polysaccharide increased the production of NO, and the level of mRNA expression of inducible nitric oxide synthase in a dose-response manner. G. atrum polysaccharide also dose-dependently induced the release of TNF-α and interleukin-1β. Generation of reactive oxygen species was promoted by PSG-1, as determined by flow cytometry. Moreover, PSG-1 induced nuclear factor-κB activation by elevation of p65 nuclear translocation, suggesting that PSG-1 probably stimulated macrophage activities by activating the nuclear factor-κB pathway.
本研究旨在评估纯化的灵芝多糖（PSG-1）对 RAW264.7 巨噬细胞系的免疫调节作用。通过异硫氰酸荧光素右旋糖苷内化的吞噬试验表明，PSG-1 刺激了巨噬细胞的吞噬作用。灵芝多糖以剂量反应方式增加了 NO 的产生和诱导型一氧化氮合酶的 mRNA 表达水平。灵芝多糖还剂量依赖性地诱导 TNF-α和白细胞介素-1β的释放。通过流式细胞术测定，PSG-1 促进了活性氧的产生。此外，PSG-1 通过提高 p65 核易位来诱导核因子-κB 的激活，表明 PSG-1 可能通过激活核因子-κB 途径刺激巨噬细胞的活性。
