Nutrition and Food Technology Group, Universidad de Antioquia, Medellin 050010, Colombia.
Development and Innovation in Alternative Proteins Group (INNOVAPROT), Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicolás Cabrera, 28049 Madrid, Spain.
Int J Mol Sci. 2024 Aug 27;25(17):9290. doi: 10.3390/ijms25179290.
Amidst increasing awareness of diet-health relationships, plant-derived bioactive peptides are recognized for their dual nutritional and health benefits. This study investigates bioactive peptides released after Alcalase hydrolysis of protein from chachafruto (), a nutrient-rich South American leguminous plant, focusing on their behavior during simulated gastrointestinal digestion. Evaluating their ability to scavenge radicals, mitigate oxidative stress, and influence immune response biomarkers, this study underscores the importance of understanding peptide interactions in digestion. The greatest contribution to the antioxidant activity was exerted by the low molecular weight peptides with ORAC values for the <3 kDa fraction of HES, GD-HES, and GID-HES of 0.74 ± 0.03, 0.72 ± 0.004, and 0.56 ± 0.01 (μmol TE/mg protein, respectively). GD-HES and GID-HES exhibited immunomodulatory effects, promoting the release of NO up to 18.52 and 8.58 µM, respectively. The findings of this study highlighted the potential of chachafruto bioactive peptides in functional foods and nutraceuticals, supporting human health through dietary interventions.
在人们对饮食与健康关系的认识不断提高的背景下，植物源生物活性肽因其兼具营养和健康益处而受到关注。本研究探讨了 Alcalase 水解南美富含营养的豆类植物 chachafruto 蛋白后释放的生物活性肽，重点研究了它们在模拟胃肠道消化过程中的行为。通过评估其清除自由基、减轻氧化应激和影响免疫反应生物标志物的能力，本研究强调了理解肽在消化过程中相互作用的重要性。具有最低分子量的肽对 HES、GD-HES 和 GID-HES 的<3 kDa 部分的 ORAC 值贡献最大，分别为 0.74±0.03、0.72±0.004 和 0.56±0.01（μmol TE/mg 蛋白）。GD-HES 和 GID-HES 表现出免疫调节作用，分别促进 NO 的释放高达 18.52 和 8.58 μM。本研究结果强调了 chachafruto 生物活性肽在功能性食品和营养保健品中的潜力，通过饮食干预支持人类健康。
