Alexandrov Sergey A, Uttam Shikhar, Bista Rajan K, Zhao Chengquan, Liu Yang
Biomedical Optical Imaging Laboratory (BOIL), Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15232, USA.
Opt Express. 2012 Apr 9;20(8):9203-14. doi: 10.1364/OE.20.009203.
We demonstrate a novel approach for the real time visualization and quantification of the 3D spatial frequencies in an image domain. Our approach is based on the spectral encoding of spatial frequency principle and permits the formation of an image as a color map in which spatially separated spectral wavelengths correspond to the dominant 3D spatial frequencies of the object. We demonstrate that our approach can visualize and analyze the dominant axial internal structure for each image point in real time and with nanoscale sensitivity to structural changes. Computer modeling and experimental results of instantaneous color visualization and quantification of 3D structures of a model system and biological samples are presented.
我们展示了一种在图像域中对三维空间频率进行实时可视化和量化的新方法。我们的方法基于空间频率原理的光谱编码，允许将图像形成为彩色图谱，其中空间分离的光谱波长对应于物体的主要三维空间频率。我们证明，我们的方法能够实时可视化和分析每个图像点的主要轴向内部结构，并对结构变化具有纳米级的敏感性。本文还给出了模型系统和生物样品三维结构的瞬时彩色可视化和量化的计算机建模及实验结果。
