空间域低相干定量相位显微镜在癌症诊断中的应用。
Spatial-domain low-coherence quantitative phase microscopy for cancer diagnosis.
机构信息
Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Pittsburgh, Pittsburgh, Pennsylvania 15232, USA.
出版信息
Opt Lett. 2010 Sep 1;35(17):2840-2. doi: 10.1364/OL.35.002840.
Abstract
A microscopy technique, spatial-domain low-coherence quantitative phase microscopy (SL-QPM) is proposed for speckle-free, quantitative phase imaging of subcellular structures with subnanometer sensitivity. We quantified, for the first time to our knowledge, the refractive index of the cell nuclei on original unmodified histology specimens. We demonstrate that the refractive index of cell nucleus is highly sensitive in detecting cancer, especially in histologically normal-appearing cells from cancer patients. Because this technique is sensitive and does not require special sample processing, it can be disseminated to all clinical settings.
摘要
一种显微镜技术，即空域低相干定量相显微镜（SL-QPM），被提出用于无散斑、具有亚纳米灵敏度的亚细胞结构定量相成像。我们首次对原始未经修饰的组织学标本中的细胞核折射率进行了量化。我们证明了细胞核折射率在癌症检测中具有高度的敏感性，尤其是在癌症患者组织学上看起来正常的细胞中。由于该技术具有敏感性且不需要特殊的样品处理，因此可以推广到所有临床环境。
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