ARC Centre of Excellence for Functional Nanomaterials, School of Chemical Engineering, The University of New South Wales, Sydney NSW 2052, Australia.
ACS Nano. 2012 May 22;6(5):4083-93. doi: 10.1021/nn3004845. Epub 2012 May 2.
Exposure to fetal bovine serum (FBS) is shown herein to reduce the aggregate size of titanium dioxide (TiO(2)) nanoparticles, affecting uptake and consequent effect on A549 and H1299 human lung cell lines. Initially, the cellular uptake of the FBS-treated TiO(2) was lower than that of non-FBS-treated TiO(2). Expulsion of particles was then observed, followed by a second phase of uptake of FBS-treated TiO(2), resulting in an increase in the cellular content of FBS-treated TiO(2), eventually exceeding the amount by cells exposed to non-FBS-treated TiO(2). Surface adsorbed vitronectin and the clathrin-mediated endocytosis pathway were shown to regulate the uptake of TiO(2) into A549 cells, while the endocytosis mechanism responsible remains elusive for H1299. Intriguingly, nystatin treatment was shown to have the unexpected effect of increasing nanoparticle uptake into the A549 cells via an alternate endocytic pathway. The surface adsorbed serum components were found to provide some protection from the cytotoxic effect of endocytosed TiO(2) nanoparticles.
本文显示，胎牛血清（FBS）的暴露会减小二氧化钛（TiO2）纳米颗粒的聚集尺寸，从而影响其摄取，并对 A549 和 H1299 人肺细胞系产生相应的影响。最初，经 FBS 处理的 TiO2 的细胞摄取量低于未经 FBS 处理的 TiO2。随后观察到颗粒被排出，然后是经 FBS 处理的 TiO2 的第二阶段摄取，导致经 FBS 处理的 TiO2 的细胞含量增加，最终超过了暴露于未经 FBS 处理的 TiO2 的细胞的含量。研究表明，表面吸附的 vitronectin 和网格蛋白介导的内吞作用途径调节了 TiO2 进入 A549 细胞的摄取，而对于 H1299，负责的内吞作用机制仍不清楚。有趣的是，制霉菌素处理显示出通过替代内吞作用途径增加 A549 细胞中纳米颗粒摄取的意外效果。发现表面吸附的血清成分对被内吞的 TiO2 纳米颗粒的细胞毒性作用提供了一定的保护。
