Golubeva N V, Podobed O V, Kukharenko V I, Del'vig A A
Vopr Med Khim. 1990 Sep-Oct;36(5):41-3.
Degradation of short- and long-life proteins was studied in human embryonal fibroblasts with normal karyotype, trisomy and triploidy. Degradation of both short- and long-life proteins by means of lysosomal enzymes was elevated in fibroblasts with anomalous karyotype at the logarithmic phase of growth as compared with normal state, when lysosomotropic agent NH4Cl was used. In transition to stationary phase of growth participation of the lysosomal system in degradation of long-life proteins was increased in fibroblasts with normal karyotype, whereas degradation of these proteins in aneuploid fibroblasts maintained at the level found during the logarithmic phase of growth. Lysosomal enzymes did not participate apparently in degradation of short-life proteins in fibroblasts with normal and anomalous karyotype at both phases of growth. The data obtained suggest that chromosomal disbalance was related to impaired regulation of protein degradation as well as to possible alteration in the ratio of cytosol and lysosomal proteolysis.
在具有正常核型、三体性和三倍体的人胚胎成纤维细胞中研究了短寿命和长寿命蛋白质的降解。当使用溶酶体促渗剂NH4Cl时，与正常状态相比，在对数生长期具有异常核型的成纤维细胞中，通过溶酶体酶对短寿命和长寿命蛋白质的降解均有所升高。在生长转变至静止期时，具有正常核型的成纤维细胞中溶酶体系统参与长寿命蛋白质降解的程度增加，而在非整倍体成纤维细胞中这些蛋白质的降解维持在对数生长期所发现的水平。在生长的两个阶段，溶酶体酶显然均不参与具有正常和异常核型的成纤维细胞中短寿命蛋白质的降解。所获得的数据表明，染色体失衡与蛋白质降解调节受损以及胞质溶胶和溶酶体蛋白水解比例的可能改变有关。
