验证 T 细胞 Xtend 试剂在不同结核危险因素个体中的 T-SPOT.TB 检测延长血液存储时间的效果。
Validation of increased blood storage times with the T-SPOT.TB assay with T-Cell Xtend reagent in individuals with different tuberculosis risk factors.
机构信息
Department of Internal Medicine, Division of Infectious Diseases, The Ohio State University, Columbus, Ohio, USA.
出版信息
J Clin Microbiol. 2012 Jul;50(7):2469-71. doi: 10.1128/JCM.00674-12. Epub 2012 Apr 18.
Abstract
We compared the performance of the T-SPOT.TB assay with blood used within 0 to 3.5 h after collection (control) to its performance with blood stored for 0 to 3.5, 5 to 8, 18 to 21, or 31 to 33 h with the addition of T-Cell Xtend (experimental), using samples from 154 participants. The 95.4% concordance between paired specimens indicated that blood can be stored for up to 33 h prior to T-SPOT.TB testing.
摘要
我们比较了 T-SPOT.TB 检测使用采集后 0 至 3.5 小时内的新鲜血样（对照组）与采集后 0 至 3.5、5 至 8、18 至 21 或 31 至 33 小时添加 T-Cell Xtend 的血样（实验组）的检测性能，实验组样本来自 154 名参与者。配对样本的 95.4%一致性表明，T-SPOT.TB 检测前血液可最多储存 33 小时。
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