Tokushima M, Sanz M L, de las Marinas M D, Oehling A
Departamento de Alergología, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Allergol Immunopathol (Madr). 1990 May-Jun;18(3):127-33.
In previous studies we described isotype-specific but antigen non-restricted soluble factors produced by human lymphocytes. In subsequent studies we demonstrated changes in the production of these factors during the course of immunotherapy (IT) by testing them on lymphocytes from normal healthy controls and allergic patients. Through these studies we confirmed that there exists a difference in lymphocytes' responsiveness to soluble factors between both groups. In this report, we investigated the effect of soluble factors on lymphocytes from allergic patients without IT (LyG1) and with IT longer than 2 years (LyG4). Peripheral blood samples were collected from healthy controls and allergic patients at different time periods of IT, and bidirectional mixed cultures were performed with the isolated lymphocytes. Supernatants obtained from chromatography were tested on lymphocytes of allergic patients without IT and with IT greater than 2 years to determine their effect on IgE synthesis. Long periods of IT reduce the production of Suppressor Factors (SF) by allergic patients as well as their responsiveness. Long periods of IT increase the responsiveness of lymphocytes of allergic patients to Enhancing Factors (EF) and decrease EF production. We propose a "receptor hypothesis" to explain these events.
在先前的研究中，我们描述了人类淋巴细胞产生的同型特异性但抗原非限制性的可溶性因子。在随后的研究中，我们通过对正常健康对照者和过敏患者的淋巴细胞进行检测，证实了在免疫治疗（IT）过程中这些因子产生的变化。通过这些研究，我们确认两组淋巴细胞对可溶性因子的反应性存在差异。在本报告中，我们研究了可溶性因子对未经免疫治疗的过敏患者（LyG1）和免疫治疗超过2年的过敏患者（LyG4）的淋巴细胞的影响。在免疫治疗的不同时间段，从健康对照者和过敏患者采集外周血样本，并对分离出的淋巴细胞进行双向混合培养。对经色谱法获得的上清液在未经免疫治疗的过敏患者和免疫治疗超过2年的过敏患者的淋巴细胞上进行检测，以确定它们对IgE合成的影响。长期免疫治疗会降低过敏患者抑制因子（SF）的产生及其反应性。长期免疫治疗会增加过敏患者淋巴细胞对增强因子（EF）的反应性并降低EF的产生。我们提出一个“受体假说”来解释这些现象。
