de las Marinas M D, Sanz M L, Diéguez I, Oehling A
Department of Allergology and Clinical Immunology, Faculty of Medicine, University of Navarra, Pamplona, Spain.
J Investig Allergol Clin Immunol. 1993 Jan-Feb;3(1):40-4.
The isotype-specific mechanisms involved in IgE synthesis are a sophisticated system about which very little is known. Several research groups have demonstrated that there is an enhancing factor (EF) in lymphocyte supernatants which is related to the regulation of the synthesis of IgE, and the influence medium-long-term immunotherapy (IT) exerts on it. Following this line of research, we studied the influence of long-term IT (> 3 years) and the presence of interleukin-4 (IL-4), not only in the different fractions where EF is contained, but also in lymphocyte supernatants after stimulation. Bidirectional mixed lymphocyte cultures were performed between lymphocytes from healthy controls (n = 62) and allergic patients (n = 98) after different periods of IT. The supernatants obtained were submitted to affinity chromatography and the second fractions, containing EF, were added to healthy control lymphocytes (n = 11) in order to test their enhancing effect on IgE synthesis. Modifications in EF production were observed, depending on the duration of IT. There were no detectable levels of IL-4 either in EF fractions or supernatants from stimulated lymphocytes.
参与IgE合成的同种型特异性机制是一个复杂的系统，目前人们对其了解甚少。几个研究小组已经证明，淋巴细胞上清液中存在一种增强因子（EF），它与IgE合成的调节以及中长期免疫疗法（IT）对其的影响有关。沿着这条研究路线，我们不仅研究了长期IT（>3年）和白细胞介素-4（IL-4）的存在对含有EF的不同组分的影响，还研究了它们对刺激后淋巴细胞上清液的影响。在不同时期的IT后，对健康对照者（n = 62）和过敏患者（n = 98）的淋巴细胞进行双向混合淋巴细胞培养。将获得的上清液进行亲和层析，并将含有EF的第二组分添加到健康对照淋巴细胞（n = 11）中，以测试它们对IgE合成的增强作用。观察到EF产生的变化取决于IT的持续时间。在EF组分或刺激淋巴细胞的上清液中均未检测到IL-4水平。
