Tokushima M, Sanz M L, de las Marinas M D, Fernández J F, Oehling A
Departamento de Alergología, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Allergol Immunopathol (Madr). 1990 Mar-Apr;18(2):63-8.
IgE synthesis is controlled not only by antigen-specific mechanism but also by other factors which selectively affect the IgE isotype. Several groups demonstrated that factors released by lymphocytes were involved in this regulation. Our previous investigations were also directed in this field. Immunotherapy improves clinical symptoms in allergic respiratory diseases and produces cellular and humoral changes that affect IgE production. So we decided to investigate if immunotherapy had any influence in the production of soluble factors. Bidirectional mixed cultures were performed with lymphocytes from healthy controls and allergic patients with different time periods of IT, and the supernatants obtained from chromatography were tested to determine their effect on IgE synthesis of normal lymphocytes. IT time periods exerted influence on the production of enhancing factors. Suppressor factors derived from allergic patients had no effect on IgE synthesis of normal lymphocytes.
IgE的合成不仅受抗原特异性机制控制，还受其他选择性影响IgE同种型的因素控制。几组研究表明，淋巴细胞释放的因子参与了这种调节。我们之前的研究也针对这一领域。免疫疗法可改善过敏性呼吸道疾病的临床症状，并产生影响IgE产生的细胞和体液变化。因此，我们决定研究免疫疗法是否对可溶性因子的产生有任何影响。对来自健康对照者和接受不同疗程免疫疗法的过敏患者的淋巴细胞进行双向混合培养，并对从色谱法获得的上清液进行检测，以确定它们对正常淋巴细胞IgE合成的影响。免疫疗法疗程对增强因子的产生有影响。来自过敏患者的抑制因子对正常淋巴细胞的IgE合成没有影响。
