Steinbock Lorenz J, Keyser Ulrich F
Cavendish Laboratory, University of Cambridge, Cambridge, UK.
Methods Mol Biol. 2012;870:135-45. doi: 10.1007/978-1-61779-773-6_8.
Small holes in membranes or nanocapillaries can be employed to detect single molecules in solution. In fact, the resistive-pulse technique based on nanopores allows for determination of length, charge, and folding state of deoxyribonucleic acid (DNA). Here, we describe the experimental procedures necessary for measuring single DNA molecules in nanocapillaries. We also discuss the measures for data analysis and how to determine that only single molecule events are observed.
膜或纳米毛细管中的小孔可用于检测溶液中的单分子。事实上，基于纳米孔的电阻脉冲技术能够测定脱氧核糖核酸（DNA）的长度、电荷和折叠状态。在此，我们描述了在纳米毛细管中测量单个DNA分子所需的实验步骤。我们还讨论了数据分析的方法以及如何确定观察到的只是单分子事件。
