Department of Chemical Engineering and Pharmaceutical Technology, University of La Laguna, 38200 La Laguna, Spain.
J Biomed Mater Res A. 2012 Sep;100(9):2382-91. doi: 10.1002/jbm.a.34183. Epub 2012 Apr 24.
Bone regeneration and vascularization with porous PLGA scaffolds loaded with VEGF (0.35 and 1.75 μg) and BMP-2 (3.5 and 17.5 μg), incorporated in PLGA microspheres, or the combination of either dose of BMP-2 with the low dose of VEGF were investigated in an intramedullary femur defect in rabbits. The system was designed to control growth factor (GF) release and maintain the GFs localized within the defect. An incomplete release was observed in vitro whereas in vivo VEGF and BMP-2 were totally delivered during 3 and 4 weeks, respectively. A weak synergistic effect of the dual delivery of VEGF and BMP-2 (high dose) was found by 4 weeks. However, the absence of an apparent synergistic long-term effect (12 weeks) of the combination over BMP-2 alone suggests that more work has to be done to optimize VEGF dose, sequential presentation, and the ratio of the two GFs to obtain a beneficial bone repair response.
用载有 VEGF（0.35 和 1.75μg）和 BMP-2（3.5 和 17.5μg）的多孔 PLGA 支架与 PLGA 微球结合，或用两种剂量的 BMP-2 与低剂量的 VEGF 组合，研究了兔骨髓内股骨缺损中的骨再生和血管化。该系统旨在控制生长因子（GF）的释放并将 GFs 保持在缺陷部位。体外观察到不完全释放，而体内 VEGF 和 BMP-2 分别在 3 周和 4 周内完全释放。4 周时发现 VEGF 和 BMP-2（高剂量）双重递送具有微弱的协同作用。然而，组合在 12 周时没有显示出明显的长期协同作用（优于 BMP-2 单独使用），这表明需要进一步优化 VEGF 剂量、顺序呈现以及两种 GFs 的比例，以获得有益的骨修复反应。
