Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China.
Biol Trace Elem Res. 2012 Nov;149(2):212-8. doi: 10.1007/s12011-012-9409-6. Epub 2012 Apr 14.
The purpose of this 42-day study was to examine the effect of dietary vanadium on intestinal microorganism diversity in the duodenum, ileum, cecum, and rectum segments of broilers by the plate count and polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE). A total of 420 1-day-old avian broilers were divided into six groups and fed on a control diet or the same diet supplemented with vanadium at the doses of 5, 15, 30, 45, and 60 mg/kg in the form of ammonium metavanadate. In comparison with control group, the dietary vanadium at the doses of 45 and 60 mg/kg could decrease the counts of Bifidobacterium spp. in the intestinal tract at 21 and 42 days of age. With increasing level in dietary vanadium, the counts of Escherichia coli were significantly increased in the ileum, cecum, and rectum and were decreased in the duodenum at 21 and 42 days of age. However, the counts of Lactobacilli were decreased in the cecum and rectum and increased in the ileum of 45 and 60 mg/kg groups. The colonization of these three bacteria could be affected by dietary vanadium. DGGE analysis showed that the number of bands in duodenum, ileum, cecum, and rectum were obviously decreased in the 30, 45, and 60 mg/kg groups at 21 and 42 days of age. In conclusion, the dietary vanadium in excess of 30 mg/kg could alter the amount and diversity of intestinal bacteria in broilers, implying that the structure and initial balance in the intestinal microbiota were disrupted.
本 42 天研究旨在通过平板计数和聚合酶链反应-变性梯度凝胶电泳（DGGE）研究饲粮钒对肉鸡十二指肠、空肠、回肠和直肠段肠道微生物多样性的影响。将 420 只 1 日龄禽肉鸡分为 6 组，分别饲喂基础饲粮或添加 5、15、30、45 和 60mg/kg 五氧化二钒的饲粮，五氧化二钒以正钒酸铵的形式添加。与对照组相比，饲粮钒添加水平为 45 和 60mg/kg 时，可降低 21 和 42 日龄肉鸡肠道双歧杆菌的数量。随着饲粮钒添加水平的增加，21 和 42 日龄时，回肠、盲肠和直肠中大肠杆菌的数量显著增加，而十二指肠中大肠杆菌的数量减少。然而，45 和 60mg/kg 组的盲肠和直肠中乳酸杆菌的数量减少，空肠中乳酸杆菌的数量增加。这三种细菌的定植可能受到饲粮钒的影响。DGGE 分析显示，21 和 42 日龄时，30、45 和 60mg/kg 组的十二指肠、空肠、回肠和直肠的条带数量明显减少。综上所述，饲粮钒超过 30mg/kg 会改变肉鸡肠道细菌的数量和多样性，这表明肠道微生物群的结构和初始平衡被破坏。
