Sudha M, Gowri R Shyamala, Prabhavathi P, Astapriya P, Devi S Yamuna, Saranya A
Department of Microbiology and Biochemistry, Nadar Saraswathi Arts and Science College, Theni, Tamil Nadu, India.
Pak J Biol Sci. 2012 Jan 1;15(1):39-43. doi: 10.3923/pjbs.2012.39.43.
Indole Acetic Acid (IAA) producing bacterium was isolated from the Rhizosphere soil and identified as Rhizobium sp. and Bacillus sp., Optimization of Indole acetic acid production was carried out at different cultural conditions, such as pH, temperature and substrate with Rhizobium sp., Bacillus sp. and Rhizobium sp., produced higher amount of Indole acetic acid (6.1 mg mL(-1)) than the Bacillus sp., (4.4 mg mL(-1)) at pH 7 and 37 degrees C in the Bengal gram substrate. Partial purification of Indole acetic acid was done by Thin Layer Chromatography (TLC). In conclusion Rhizobium sp., appear to be a suitable soil microorganism for high level of IAA production.
从根际土壤中分离出产生吲哚乙酸（IAA）的细菌，并鉴定为根瘤菌属和芽孢杆菌属。在不同培养条件下，如pH值、温度和底物，对根瘤菌属、芽孢杆菌属进行吲哚乙酸产量优化。在孟加拉鹰嘴豆底物中，pH值为7、温度为37℃时，根瘤菌属产生的吲哚乙酸量（6.1 mg mL(-1)）高于芽孢杆菌属（4.4 mg mL(-1)）。通过薄层色谱法（TLC）对吲哚乙酸进行了部分纯化。总之，根瘤菌属似乎是一种适合高产IAA的土壤微生物。
