Chandra Sheela, Askari Kazim, Kumari Madhumita
Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India.
Deptt. of Biotechnology, Era University, Lucknow, India.
J Genet Eng Biotechnol. 2018 Dec;16(2):581-586. doi: 10.1016/j.jgeb.2018.09.001. Epub 2018 Dec 8.
The ability to synthesize Indole-3-acetic acid (IAA) is widely associated with the plant growth promoting rhizobacteria (PGPR). The present work deals with isolation and characterization of such bacteria from the rhizosphere of medicinal plant and optimization of IAA production from its isolates. The optimization of IAA production was carried out at different pH and temperature with varied carbon and nitrogen sources of culture media. Out of different isolates obtained, three of them were screened as efficient PGPRs on the basis of different plant growth promoting attributes. Isolates CA1001 and CA2004 showed better production of IAA at pH 9 (91.7 µg ml) and at temperature 37 °C (81.7 µg ml). Dextrose (1%) was found to be the best carbon source for isolate CA1001 with 104 µg ml IAA production. Isolate CA 2004 showed best production of IAA 36 µg ml and 34 µg ml at 1.5% and 1% Beef extract as nitrogen source respectively. Isolate CA 1001 showed 32 µg ml IAA production at 0.5% nicotinic acid concentration. From the current study, CA1001 and CA2004 emerged as noble alternatives for IAA production further which also resulted in root and shoot biomass generation in crop plants, hence can be further used as bio-inoculants for plant growth promotion.
合成吲哚 - 3 - 乙酸（IAA）的能力与促进植物生长的根际细菌（PGPR）广泛相关。本研究致力于从药用植物根际分离和鉴定此类细菌，并优化其分离株的IAA产量。在不同的pH值和温度下，使用不同的碳源和氮源培养基对IAA产量进行优化。在获得的不同分离株中，基于不同的促进植物生长特性，筛选出其中三株作为高效的PGPR。分离株CA1001和CA2004在pH 9（91.7μg/ml）和温度37°C（81.7μg/ml）时显示出更好的IAA产量。葡萄糖（1%）被发现是分离株CA1001产生IAA的最佳碳源，产量为104μg/ml。分离株CA 2004分别以1.5%和1%的牛肉提取物作为氮源时，IAA产量最高，分别为36μg/ml和34μg/ml。分离株CA 1001在烟酸浓度为0.5%时IAA产量为32μg/ml。从当前研究来看，CA1001和CA2004成为进一步生产IAA的优质替代物，这也导致了作物植物根和地上部分生物量的增加，因此可进一步用作促进植物生长的生物接种剂。
