School of Chemical & Biological Engineering, Bio-Max Institute, Seoul National University, 599 Daehakdong, Gwanak-gu, Seoul 151-744, Republic of Korea.
Integr Biol (Camb). 2012 Jun;4(6):587-94. doi: 10.1039/c2ib20017a. Epub 2012 Apr 25.
In order to successfully utilize stem cells for therapeutic applications in regenerative medicine, efficient differentiation into a specific cell lineage and guidance of axons in a desired direction is crucial. Here, we used aligned multi-walled carbon nanotube (MWCNT) sheets to differentiate human mesenchymal stem cells (hMSCs) into neural cells. Human MSCs present a preferential adhesion to aligned CNT sheets with longitudinal stretch parallel to the CNT orientation direction. Cell elongation was 2-fold higher than the control and most of the cells were aligned on CNT sheets within 5° from the CNT orientation direction. Furthermore, a significant, synergistic enhancement of neural differentiation was observed in hMSCs cultured on the CNT sheets. Axon outgrowth was also controlled using nanoscale patterning of CNTs. This CNT sheet provides a new cellular scaffold platform that can regulate morphogenesis and differentiation of stem cells, which could open up a new approach for tissue and stem cell regeneration.
为了在再生医学的治疗应用中成功利用干细胞，将其高效分化为特定的细胞谱系并引导轴突朝期望的方向生长至关重要。在这里，我们使用定向排列的多壁碳纳米管（MWCNT）片将人骨髓间充质干细胞（hMSC）分化为神经细胞。人 MSC 优先附着于定向 CNT 片上，其纵向拉伸与 CNT 取向方向平行。细胞的伸长率是对照组的两倍，并且大多数细胞在与 CNT 取向方向相差 5°以内的 CNT 片上排列。此外，在 CNT 片上培养的 hMSC 中观察到神经分化显著协同增强。通过 CNT 的纳米级图案化还可以控制轴突的生长。这种 CNT 片提供了一种新的细胞支架平台，可调节干细胞的形态发生和分化，这为组织和干细胞再生开辟了新途径。
