Han Lin, Zhou Jing, Sun Yubing, Zhang Yu, Han Jung, Fu Jianping, Fan Rong
Biomedical Engineering, Yale University , Malone Center Room / space 103C , 55 Prospect Street , New Haven, CT 06511 e-mail:
Anesthesiology, Yale School of Medicine, Biomedical Engineering, Yale University , Room 314 , 10 Amistad Street , New Haven, CT 06510 e-mail:
J Nanotechnol Eng Med. 2014 Nov;5(4):0410041-410049. doi: 10.1115/1.4030615.
Single-crystalline nanoporous gallium nitride (GaN) thin films were fabricated with the pore size readily tunable in 20-100 nm. Uniform adhesion and spreading of human mesenchymal stem cells (hMSCs) seeded on these thin films peak on the surface with pore size of 30 nm. Substantial cell elongation emerges as pore size increases to ∼80 nm. The osteogenic differentiation of hMSCs occurs preferentially on the films with 30 nm sized nanopores, which is correlated with the optimum condition for cell spreading, which suggests that adhesion, spreading, and stem cell differentiation are interlinked and might be coregulated by nanotopography.
制备出了孔径易于在20 - 100nm范围内调节的单晶纳米多孔氮化镓（GaN）薄膜。接种在这些薄膜上的人间充质干细胞（hMSCs）的均匀黏附与铺展在孔径为30nm的表面达到峰值。随着孔径增加到约80nm，细胞出现明显伸长。hMSCs的成骨分化优先发生在具有30nm大小纳米孔的薄膜上，这与细胞铺展的最佳条件相关，这表明黏附、铺展和干细胞分化相互关联，可能由纳米拓扑结构共同调节。
