School of Medicine, Shaoxing University, Shaoxing, PR China.
J Biomater Appl. 2013 Aug;28(2):232-40. doi: 10.1177/0885328212444642. Epub 2012 Apr 24.
Fluorescence imaging technique has been used for imaging of biological cells and tissues in vivo. The Cd-free luminescent quantum dots conjugating with a cancer targeting ligand has been taken as a promising biocompatibility and low cytotoxicity system for targeted cancer imaging. This work reports the synthesis of fluorescent-doped core/shell quantum dots of water-soluble manganese-doped zinc sulfide. Quantum dots of manganese-doped zinc sulfide were prepared by nucleation doping strategy, with 3-mercaptopropionic acid as stabilizer at 90 in aqueous solution. The manganese-doped zinc sulfide nanoparticles exhibit strong orange fluorescence under UV irradiation, resistance to photo-bleaching, and low-cytotoxicity to HeLa cells. The structure and optical properties of nanoparticles were characterized by scanning electron microscope, X-ray diffraction, dynamic light scattering, and photoluminescence emission spectroscopy. Manganese-doped zinc sulfide nanoparticles conjugated with folic acid using 2,2'-(ethylenedioxy)-bis-(ethylamine) as the linker. The covalent binding of both 2,2'-(ethylenedioxy)-bis-(ethylamine) and folic acid on the surface of manganese-doped zinc sulfide nanoparticles probed by Fourier transform infrared spectroscopy detection. Furthermore, in vitro cytotoxicity assessment of manganese-doped zinc sulfide-folic acid probes use HeLa cells. The obtained fluorescent probes (manganese-doped zinc sulfide) were used for tumor targeting and imaging in vivo. The manganese-doped zinc sulfide-folic acid fluorescent probes which targeting the tumor cells in the body of nude mouse tumor model would emit orange fluorescence, when exposed to a 365 nm lamp. We investigate the biodistribution of the manganese-doped zinc sulfide-folic acid fluorescent probes in tumor mouse model by measuring zinc concentration in tissues. These studies demonstrate the practicality of manganese-doped zinc sulfide-folic acid fluorescent probes as promising platform for tumor targeting and imaging in vivo.
荧光成像技术已被用于活体生物细胞和组织的成像。将与癌症靶向配体缀合的无镉发光量子点用作具有良好生物相容性和低细胞毒性的靶向癌症成像系统。本工作报道了水溶性锰掺杂硫化锌荧光掺杂核/壳量子点的合成。通过成核掺杂策略，以 3-巯基丙酸为稳定剂，在 90℃的水溶液中制备了锰掺杂硫化锌量子点。锰掺杂硫化锌纳米粒子在紫外光照射下呈现强橙色荧光，具有抗光漂白性，对 HeLa 细胞的细胞毒性低。通过扫描电子显微镜、X 射线衍射、动态光散射和光致发光发射光谱对纳米粒子的结构和光学性质进行了表征。使用 2,2'-(乙二氧基)-双-(乙胺)作为连接体将叶酸与锰掺杂硫化锌纳米粒子偶联。傅里叶变换红外光谱检测证实了表面上锰掺杂硫化锌纳米粒子与 2,2'-(乙二氧基)-双-(乙胺)和叶酸的共价结合。此外，通过 HeLa 细胞评估了锰掺杂硫化锌-叶酸探针的体外细胞毒性。所得荧光探针（锰掺杂硫化锌）用于体内肿瘤靶向和成像。当将锰掺杂硫化锌-叶酸荧光探针暴露于 365nm 灯时，体内裸鼠肿瘤模型中的肿瘤细胞会发出橙色荧光。我们通过测量组织中的锌浓度来研究锰掺杂硫化锌-叶酸荧光探针在肿瘤小鼠模型中的体内分布。这些研究表明，锰掺杂硫化锌-叶酸荧光探针作为体内肿瘤靶向和成像的有前途的平台具有实用性。
