腺嘌呤核苷酸对烟碱型乙酰胆碱受体功能的调节
Regulation of nicotinic acetylcholine receptor function by adenine nucleotides.
作者信息
Eterović V A, Li L, Palma A, McNamee M G
机构信息
Department of Biochemistry, Universidad Central del Caribe, Cayey, Puerto Rico 00634.
出版信息
Cell Mol Neurobiol. 1990 Sep;10(3):423-33. doi: 10.1007/BF00711184.
Abstract
- Nicotinic acetylcholine receptors (nAChR)4 from BC3H1 cells (which express a skeletal muscle-type receptor) and from Torpedo californica electric organ were expressed in Xenopus laevis oocytes and studied with a voltage-clamp technique. 2. We found that bath application of ATP in the micromolar to millimolar range increased the ACh-elicited current in both muscle and electrocyte receptors. The effect of ATP increased with successive applications. This "use-dependent" increase in potentiation was Ca2+ dependent, while the potentiation itself was not. 3. Four other nucleotides were tested on muscle nAChR: ADP, AMP, adenosine, and GTP. Of these, only ADP was a potentiator, but its effect was not use dependent. Neither ATP nor ADP affected the resting potential of the oocyte membrane. 4. ADP potentiated the response to suberyldicholine and nicotine, as well as ACh. 5. Finally, ADP reversed the phencyclidine-induced block of ACh currents in oocytes expressing muscle nAChR.
摘要
- 来自BC3H1细胞（表达骨骼肌型受体）和加州电鳐电器官的烟碱型乙酰胆碱受体（nAChR）4在非洲爪蟾卵母细胞中表达，并采用电压钳技术进行研究。2. 我们发现，在微摩尔到毫摩尔范围内浴加ATP可增加肌肉和电细胞受体中由乙酰胆碱引发的电流。ATP的效应随着连续应用而增强。这种“使用依赖性”的增强作用依赖于Ca2+，而增强作用本身并不依赖于Ca2+。3. 对肌肉nAChR测试了其他四种核苷酸：ADP、AMP、腺苷和GTP。其中，只有ADP是一种增强剂，但其效应不具有使用依赖性。ATP和ADP均不影响卵母细胞膜的静息电位。4. ADP增强了对辛二酰胆碱、尼古丁以及乙酰胆碱的反应。5. 最后，ADP逆转了苯环利定对表达肌肉nAChR的卵母细胞中乙酰胆碱电流的阻断作用。
相似文献
1
Regulation of nicotinic acetylcholine receptor function by adenine nucleotides.腺嘌呤核苷酸对烟碱型乙酰胆碱受体功能的调节
Cell Mol Neurobiol. 1990 Sep;10(3):423-33. doi: 10.1007/BF00711184.
2
Differential effects of dimethyl sulfoxide on nicotinic acetylcholine receptors from mouse muscle and Torpedo electrocytes.
Neurosci Lett. 1997 Jul 25;230(3):163-6. doi: 10.1016/s0304-3940(97)00505-3.
3
Determinants of phencyclidine potency on the nicotinic acetylcholine receptors from muscle and electric organ.苯环利定对肌肉和电鳐电器官烟碱型乙酰胆碱受体作用强度的决定因素。
Cell Mol Neurobiol. 1999 Dec;19(6):745-57. doi: 10.1023/a:1006905106834.
4
Modulation of nicotinic acetylcholine receptor channel by pH: a difference in pH sensitivity of Torpedo and mouse receptors expressed in Xenopus oocytes.pH对烟碱型乙酰胆碱受体通道的调节：非洲爪蟾卵母细胞中表达的电鳐和小鼠受体的pH敏感性差异
Cell Mol Neurobiol. 1992 Apr;12(2):83-93. doi: 10.1007/BF00713363.
5
The acetylcholinesterase inhibitor BW284c51 is a potent blocker of Torpedo nicotinic AchRs incorporated into the Xenopus oocyte membrane.乙酰胆碱酯酶抑制剂BW284c51是掺入非洲爪蟾卵母细胞膜中的电鳐烟碱型乙酰胆碱受体的有效阻滞剂。
Br J Pharmacol. 2005 Jan;144(1):88-97. doi: 10.1038/sj.bjp.0705965.
6
Blockage of mouse muscle nicotinic receptors by serotonergic compounds.血清素类化合物对小鼠肌肉烟碱型受体的阻断作用。
Exp Physiol. 1999 Sep;84(5):847-64.
7
Effect of galantamine on the human alpha7 neuronal nicotinic acetylcholine receptor, the Torpedo nicotinic acetylcholine receptor and spontaneous cholinergic synaptic activity.加兰他敏对人α7神经元烟碱型乙酰胆碱受体、电鳐烟碱型乙酰胆碱受体及自发性胆碱能突触活动的影响。
Br J Pharmacol. 2005 Jul;145(5):672-8. doi: 10.1038/sj.bjp.0706221.
8
Effects of cannabidiol on the function of α7-nicotinic acetylcholine receptors.大麻二酚对α7 型烟碱型乙酰胆碱受体功能的影响。
Eur J Pharmacol. 2013 Nov 15;720(1-3):310-9. doi: 10.1016/j.ejphar.2013.10.011. Epub 2013 Oct 18.
9
Oleic acid enhances ACh receptor currents by activation of Ca2+/calmodulin-dependent protein kinase II.油酸通过激活钙/钙调蛋白依赖性蛋白激酶II增强乙酰胆碱受体电流。
Neuroreport. 1997 Feb 10;8(3):597-601. doi: 10.1097/00001756-199702100-00004.
10
[Functional interaction between nicotinic cholinergic receptors and Na, K-ATPase in the skeletal muscles].[骨骼肌中烟碱型胆碱能受体与钠钾ATP酶之间的功能相互作用]
Ross Fiziol Zh Im I M Sechenova. 2004 Jan;90(1):59-72.
引用本文的文献
1
Action of suramin upon ecto-apyrase activity and synaptic depression of Torpedo electric organ.苏拉明对电鳐电器官外切-ATP酶活性及突触抑制的作用。
Br J Pharmacol. 1996 Jul;118(5):1232-6. doi: 10.1111/j.1476-5381.1996.tb15528.x.
2
Diterpenoids from Caribbean gorgonians act as noncompetitive inhibitors of the nicotinic acetylcholine receptor.来自加勒比海柳珊瑚的二萜类化合物可作为烟碱型乙酰胆碱受体的非竞争性抑制剂。
Cell Mol Neurobiol. 1993 Apr;13(2):99-110. doi: 10.1007/BF00735367.
3
Potentiation by ATP of the postsynaptic acetylcholine response at developing neuromuscular synapses in Xenopus cell cultures.三磷酸腺苷（ATP）对非洲爪蟾细胞培养物中发育中的神经肌肉突触后乙酰胆碱反应的增强作用。
J Physiol. 1994 Jun 15;477 ( Pt 3)(Pt 3):449-58. doi: 10.1113/jphysiol.1994.sp020206.
4
Electric organ polyamines and their effects on the acetylcholine receptor.电鳐电器官中的多胺及其对乙酰胆碱受体的影响。
Cell Mol Neurobiol. 1992 Apr;12(2):95-106. doi: 10.1007/BF00713364.
本文引用的文献
1
Increase of acetylcholine-receptor sensitivity by adenosine triphosphate: a novel action of ATP on ACh-sensitivity.三磷酸腺苷提高乙酰胆碱受体敏感性：ATP对ACh敏感性的一种新作用。
Br J Pharmacol. 1981 Oct;74(2):505-7. doi: 10.1111/j.1476-5381.1981.tb09997.x.
2
Mechanism of phencyclidine binding to the acetylcholine receptor from Torpedo electroplaque.
Mol Pharmacol. 1984 May;25(3):360-8.
3
Modulatory actions of ATP on membrane potentials of bullfrog sympathetic ganglion cells.三磷酸腺苷对牛蛙交感神经节细胞膜电位的调节作用。
Brain Res. 1983 Jan 10;258(2):313-7. doi: 10.1016/0006-8993(83)91157-5.
4
Adenosine-induced slow ionic currents in the Xenopus oocyte.非洲爪蟾卵母细胞中腺苷诱导的慢离子电流。
Nature. 1982 Aug 5;298(5874):572-4. doi: 10.1038/298572a0.
5
ATP-evoked membrane responses in Xenopus oocytes.非洲爪蟾卵母细胞中ATP诱发的膜反应。
Pflugers Arch. 1986 Feb;406(2):158-62. doi: 10.1007/BF00586677.
6
Location of functional regions of acetylcholine receptor alpha-subunit by site-directed mutagenesis.通过定点诱变确定乙酰胆碱受体α亚基功能区的位置。
Nature. 1985;313(6001):364-9. doi: 10.1038/313364a0.
7
Localization of azidophencyclidine-binding site on the nicotinic acetylcholine receptor alpha-subunit.叠氮苯环己哌啶结合位点在烟碱型乙酰胆碱受体α亚基上的定位。
Biochem Biophys Res Commun. 1987 Jun 15;145(2):810-6. doi: 10.1016/0006-291x(87)91037-0.
8
Extracellular ATP: effects, sources and fate.细胞外ATP：作用、来源及去向
Biochem J. 1986 Jan 15;233(2):309-19. doi: 10.1042/bj2330309.
9
Effect of adenosine triphosphate on the sensitivity of the nicotinic acetylcholine-receptor in the bullfrog sympathetic ganglion cell.三磷酸腺苷对牛蛙交感神经节细胞烟碱型乙酰胆碱受体敏感性的影响。
Br J Pharmacol. 1985 Feb;84(2):525-31. doi: 10.1111/j.1476-5381.1985.tb12937.x.
10
Modulation of neuronal signal transduction systems by extracellular ATP.细胞外ATP对神经元信号转导系统的调节作用。
J Neurochem. 1988 Jan;50(1):295-301. doi: 10.1111/j.1471-4159.1988.tb13263.x.
Zotero 插件