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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Eterović V A, Li L, Palma A, McNamee M G

Department of Biochemistry, Universidad Central del Caribe, Cayey, Puerto Rico 00634.

Cell Mol Neurobiol. 1990 Sep;10(3):423-33. doi: 10.1007/BF00711184.

Nicotinic acetylcholine receptors (nAChR)4 from BC3H1 cells (which express a skeletal muscle-type receptor) and from Torpedo californica electric organ were expressed in Xenopus laevis oocytes and studied with a voltage-clamp technique. 2. We found that bath application of ATP in the micromolar to millimolar range increased the ACh-elicited current in both muscle and electrocyte receptors. The effect of ATP increased with successive applications. This "use-dependent" increase in potentiation was Ca2+ dependent, while the potentiation itself was not. 3. Four other nucleotides were tested on muscle nAChR: ADP, AMP, adenosine, and GTP. Of these, only ADP was a potentiator, but its effect was not use dependent. Neither ATP nor ADP affected the resting potential of the oocyte membrane. 4. ADP potentiated the response to suberyldicholine and nicotine, as well as ACh. 5. Finally, ADP reversed the phencyclidine-induced block of ACh currents in oocytes expressing muscle nAChR.

来自BC3H1细胞（表达骨骼肌型受体）和加州电鳐电器官的烟碱型乙酰胆碱受体（nAChR）4在非洲爪蟾卵母细胞中表达，并采用电压钳技术进行研究。2. 我们发现，在微摩尔到毫摩尔范围内浴加ATP可增加肌肉和电细胞受体中由乙酰胆碱引发的电流。ATP的效应随着连续应用而增强。这种“使用依赖性”的增强作用依赖于Ca2+，而增强作用本身并不依赖于Ca2+。3. 对肌肉nAChR测试了其他四种核苷酸：ADP、AMP、腺苷和GTP。其中，只有ADP是一种增强剂，但其效应不具有使用依赖性。ATP和ADP均不影响卵母细胞膜的静息电位。4. ADP增强了对辛二酰胆碱、尼古丁以及乙酰胆碱的反应。5. 最后，ADP逆转了苯环利定对表达肌肉nAChR的卵母细胞中乙酰胆碱电流的阻断作用。

Eterović V A, Li L, Palma A, McNamee M G

Department of Biochemistry, Universidad Central del Caribe, Cayey, Puerto Rico 00634.

Cell Mol Neurobiol. 1990 Sep;10(3):423-33. doi: 10.1007/BF00711184.

Nicotinic acetylcholine receptors (nAChR)4 from BC3H1 cells (which express a skeletal muscle-type receptor) and from Torpedo californica electric organ were expressed in Xenopus laevis oocytes and studied with a voltage-clamp technique. 2. We found that bath application of ATP in the micromolar to millimolar range increased the ACh-elicited current in both muscle and electrocyte receptors. The effect of ATP increased with successive applications. This "use-dependent" increase in potentiation was Ca2+ dependent, while the potentiation itself was not. 3. Four other nucleotides were tested on muscle nAChR: ADP, AMP, adenosine, and GTP. Of these, only ADP was a potentiator, but its effect was not use dependent. Neither ATP nor ADP affected the resting potential of the oocyte membrane. 4. ADP potentiated the response to suberyldicholine and nicotine, as well as ACh. 5. Finally, ADP reversed the phencyclidine-induced block of ACh currents in oocytes expressing muscle nAChR.

来自BC3H1细胞（表达骨骼肌型受体）和加州电鳐电器官的烟碱型乙酰胆碱受体（nAChR）4在非洲爪蟾卵母细胞中表达，并采用电压钳技术进行研究。2. 我们发现，在微摩尔到毫摩尔范围内浴加ATP可增加肌肉和电细胞受体中由乙酰胆碱引发的电流。ATP的效应随着连续应用而增强。这种“使用依赖性”的增强作用依赖于Ca2+，而增强作用本身并不依赖于Ca2+。3. 对肌肉nAChR测试了其他四种核苷酸：ADP、AMP、腺苷和GTP。其中，只有ADP是一种增强剂，但其效应不具有使用依赖性。ATP和ADP均不影响卵母细胞膜的静息电位。4. ADP增强了对辛二酰胆碱、尼古丁以及乙酰胆碱的反应。5. 最后，ADP逆转了苯环利定对表达肌肉nAChR的卵母细胞中乙酰胆碱电流的阻断作用。