三阶段培养法提高银耳胞外多糖的产量。
A three-stage culture process for improved exopolysaccharide production by Tremella fuciformis.
机构信息
Center for Bioengineering and Biotechnology, China University of Petroleum, East China, Qingdao 266555, PR China.
出版信息
Bioresour Technol. 2012 Jul;116:526-8. doi: 10.1016/j.biortech.2012.03.117. Epub 2012 Apr 6.
Tremella fuciformis produces several bioactive secondary metabolites including exopolysaccharides. Cultivation of the fungus was carried out in a three-stage process consisting of a 1.5-day cultivation with orbital shaking at 200 rpm, a 1.5-day cultivation with reciprocal shaking at 200 strokes, and a 1.5-day cultivation with orbital shaking at 200 rpm. Exopolysaccharide production and specific production rate reached 5.80 g L(-1) and 0.15 d(-1), respectively, which is an increase of 260% and 200% compared with the corresponding values for fermentations with orbital shaking only, and of 243% and 150% compared with the corresponding values for fermentations with reciprocal shaking only. The three-stage culture method is time-saving and easy to operate.
银耳产生多种生物活性次级代谢产物，包括胞外多糖。真菌的培养采用三阶段工艺进行，包括 1.5 天的 200rpm 轨道摇床培养、1.5 天的 200 冲程往复摇床培养和 1.5 天的 200rpm 轨道摇床培养。胞外多糖产量和比生产速率分别达到 5.80 g/L 和 0.15 d(-1)，与仅采用轨道摇床培养的发酵相比，分别提高了 260%和 200%，与仅采用往复摇床培养的发酵相比，分别提高了 243%和 150%。三阶段培养方法省时且易于操作。
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