Center for Bioengineering and Biotechnology, China University of Petroleum, East China, Qingdao 266555, PR China.
Bioresour Technol. 2012 Jul;116:526-8. doi: 10.1016/j.biortech.2012.03.117. Epub 2012 Apr 6.
Tremella fuciformis produces several bioactive secondary metabolites including exopolysaccharides. Cultivation of the fungus was carried out in a three-stage process consisting of a 1.5-day cultivation with orbital shaking at 200 rpm, a 1.5-day cultivation with reciprocal shaking at 200 strokes, and a 1.5-day cultivation with orbital shaking at 200 rpm. Exopolysaccharide production and specific production rate reached 5.80 g L(-1) and 0.15 d(-1), respectively, which is an increase of 260% and 200% compared with the corresponding values for fermentations with orbital shaking only, and of 243% and 150% compared with the corresponding values for fermentations with reciprocal shaking only. The three-stage culture method is time-saving and easy to operate.
银耳产生多种生物活性次级代谢产物，包括胞外多糖。真菌的培养采用三阶段工艺进行，包括 1.5 天的 200rpm 轨道摇床培养、1.5 天的 200 冲程往复摇床培养和 1.5 天的 200rpm 轨道摇床培养。胞外多糖产量和比生产速率分别达到 5.80 g/L 和 0.15 d(-1)，与仅采用轨道摇床培养的发酵相比，分别提高了 260%和 200%，与仅采用往复摇床培养的发酵相比，分别提高了 243%和 150%。三阶段培养方法省时且易于操作。
